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Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering.

ABSTRACT: Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.

SUBMITTER: Hardy JG

PROVIDER: S-EPMC5456849 | biostudies-other | 2016 Jul

REPOSITORIES: biostudies-other

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Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering.

Hardy John G JG Torres-Rendon Jose Guillermo JG Leal-Egaña Aldo A Walther Andreas A Schlaad Helmut H Cölfen Helmut H Scheibel Thomas R TR

Materials (Basel, Switzerland) 20160711 7

Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-<i>co</i>-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with ca ...[more]

PMID: 28773681

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