Ontology highlight
ABSTRACT:
SUBMITTER: Harayama T
PROVIDER: S-EPMC5460891 | biostudies-other | 2017
REPOSITORIES: biostudies-other
Harayama Takeshi T Riezman Howard H
PloS one 20170606 6
Genome editing by the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR-associated protein 9) system is a revolutionary strategy to study gene functions. Since the efficiency of gene disruption in cell culture does not reach 100% typically, cloning of mutant cells is often performed to obtain fully mutated cells. Therefore, a method to discriminate accurately mutated clones easily and quickly is crucial to accelerate the research using CRISPR/Cas9. Here, we show tha ...[more]