Ontology highlight
ABSTRACT:
SUBMITTER: Miyama T
PROVIDER: S-EPMC5473819 | biostudies-other | 2017 Jun
REPOSITORIES: biostudies-other
Miyama Takahiko T Kawase Takakazu T Kitaura Kazutaka K Chishaki Ren R Shibata Masashi M Oshima Kumi K Hamana Hiroshi H Kishi Hiroyuki H Muraguchi Atsushi A Kuzushima Kiyotaka K Saji Hiroh H Shin-I Tadasu T Suzuki Ryuji R Ichinohe Tatsuo T
Scientific reports 20170616 1
To expand our knowledge of the ontogeny of the T-cell receptor (TCR) repertoire of antigen-specific T-cell subsets, we combined next-generation deep sequencing and single-cell multiplex clonotype analysis to evaluate the diversity and frequency of paired TCRs, their functions and whether clonotypic TCRs are shared among different individuals. Using an HLA-A*02-restricted cytomegalovirus (CMV) pp65-derived immunogenic peptide, we found that the more dominant pp65-specific TCR clonotypes in the bl ...[more]