Modulation of iTBS after-effects via concurrent directional TDCS: A proof of principle study.
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ABSTRACT:
SUBMITTER: Tremblay S
PROVIDER: S-EPMC5487022 | biostudies-other | 2017 Jul - Aug
REPOSITORIES: biostudies-other
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| S-EPMC11366644 | biostudies-literature
Project description:We have developed a multi-analyte fluid-phase protein array technology termed high-throughput immunophenotyping using transcription (HIT). This method employs a panel of monoclonal antibodies, each tagged with a unique oligonucleotide sequence that serves as a molecular barcode. After staining a sample, T7 polymerase amplifies the tags which are then hybridized to a DNA microarray for indirect measurement of each analyte. Here we coupled 44 of the tags to aliquots of an IgG1 isotype negative control antibody and the 4 remaining tags we coupled to anti-CD3, anti-CD4, anti-CD19, and anti-CD20 to create a 48-plex HIT cocktail. We then used this cocktail to stain 1 x 10^6 T or B cells and during amplification incorporated either cyanine 3-UTP (Cy3-UTP) or Cy5-UTP. Additionally, we snap froze and thawed an aliquot of the cocktail (FT). Keywords: protein profiling, cell type comparison
2008-03-08 | GSE10761 | GEO