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Expression of the murine fibroblast growth factor 5 gene in the adult central nervous system.


ABSTRACT: The murine homolog of the fibroblast growth factor-5 (FGF-5) gene has been cloned, and the sequence of the gene's three exons has been determined. The murine gene and the previously isolated human FGF-5 gene are substantially homologous within the coding sequences and in upstream sequences, which contain an additional open reading frame. We have used a portion of the murine gene as probe to detect FGF-5 RNA in adult mouse tissues by both Northern blot and in situ hybridization methods. FGF-5 RNA is present at low levels in widely distributed areas of the central nervous system. Several loci of FGF-5 expression could be localized by in situ hybridization and include portions of the cerebral cortex, hippocampus, and thalamus. Neuronal expression accounts for at least some of the FGF-5 RNA synthesized in the central nervous system.

SUBMITTER: Haub O 

PROVIDER: S-EPMC54884 | biostudies-other | 1990 Oct

REPOSITORIES: biostudies-other

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Expression of the murine fibroblast growth factor 5 gene in the adult central nervous system.

Haub O O   Drucker B B   Goldfarb M M  

Proceedings of the National Academy of Sciences of the United States of America 19901001 20


The murine homolog of the fibroblast growth factor-5 (FGF-5) gene has been cloned, and the sequence of the gene's three exons has been determined. The murine gene and the previously isolated human FGF-5 gene are substantially homologous within the coding sequences and in upstream sequences, which contain an additional open reading frame. We have used a portion of the murine gene as probe to detect FGF-5 RNA in adult mouse tissues by both Northern blot and in situ hybridization methods. FGF-5 RNA  ...[more]

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