Assessment of Single Cell RNA-Seq Normalization Methods.
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ABSTRACT:
SUBMITTER: Ding B
PROVIDER: S-EPMC5499114 | biostudies-other | 2017 Jul
REPOSITORIES: biostudies-other
Publications
Assessment of Single Cell RNA-Seq Normalization Methods.
Ding Bo B Zheng Lina L Wang Wei W
G3 (Bethesda, Md.) 20170705 7
We have assessed the performance of seven normalization methods for single cell RNA-seq using data generated from dilution of RNA samples. Our analyses showed that methods considering spike-in External RNA Control Consortium (ERCC) RNA molecules significantly outperformed those not considering ERCCs. This work provides a guidance of selecting normalization methods to remove technical noise in single cell RNA-seq data. ...[more]
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