Dataset Information

Identification of the miRNA-mRNA regulatory network of small cell osteosarcoma based on RNA-seq.

ABSTRACT: Small cell osteosarcoma (SCO) is a rare subtype of osteosarcoma characterized by highly aggressive progression and a poor prognosis. The miRNA and mRNA expression profiles of peripheral blood mononuclear cells (PBMCs) were obtained in 3 patients with SCO and 10 healthy individuals using high-throughput RNA-sequencing. We identified 37 dysregulated miRNAs and 1636 dysregulated mRNAs in patients with SCO compared to the healthy controls. Specifically, the 37 dysregulated miRNAs consisted of 27 up-regulated miRNAs and 10 down-regulated miRNAs; the 1636 dysregulated mRNAs consisted of 555 up-regulated mRNAs and 1081 down-regulated mRNAs. The target-genes of miRNAs were predicted, and 1334 negative correlations between miRNAs and mRNAs were used to construct an miRNA-mRNA regulatory network. Dysregulated genes were significantly enriched in pathways related to cancer, mTOR signaling and cell cycle signaling. Specifically, hsa-miR-26b-5p, hsa-miR-221-3p and hsa-miR-125b-2-3p were significantly dysregulated miRNAs and exhibited a high degree of connectivity with target genes. Overall, the expression of dysregulated genes in tumor tissues and peripheral blood samples of patients with SCO measured by quantitative real-time polymerase chain reaction corroborated with our bioinformatics analyses based on the expression profiles of PBMCs from patients with SCO. Thus, hsa-miR-26b-5p, hsa-miR-221-3p and hsa-miR-125b-2-3p may be involved in SCO tumorigenesis.

SUBMITTER: Xie L

PROVIDER: S-EPMC5522085 | biostudies-other | 2017 Jun

REPOSITORIES: biostudies-other

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Identification of the miRNA-mRNA regulatory network of small cell osteosarcoma based on RNA-seq.

Xie Lin L Liao Yedan Y Shen Lida L Hu Fengdi F Yu Sunlin S Zhou Yonghong Y Zhang Ya Y Yang Yihao Y Li Dongqi D Ren Minyan M Yuan Zhongqin Z Yang Zuozhang Z

Oncotarget 20170601 26

Small cell osteosarcoma (SCO) is a rare subtype of osteosarcoma characterized by highly aggressive progression and a poor prognosis. The miRNA and mRNA expression profiles of peripheral blood mononuclear cells (PBMCs) were obtained in 3 patients with SCO and 10 healthy individuals using high-throughput RNA-sequencing. We identified 37 dysregulated miRNAs and 1636 dysregulated mRNAs in patients with SCO compared to the healthy controls. Specifically, the 37 dysregulated miRNAs consisted of 27 up- ...[more]

PMID: 28477009

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Dataset Information

Identification of the miRNA-mRNA regulatory network of small cell osteosarcoma based on RNA-seq.

Publications

Identification of the miRNA-mRNA regulatory network of small cell osteosarcoma based on RNA-seq.

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