Unknown

Dataset Information

0

Molecular cloning, functional expression, and chromosomal localization of mouse hepatocyte nuclear factor 1.


ABSTRACT: The homeodomain-containing transcription factor hepatocyte nuclear factor 1 (HNF-1) most likely plays an essential role during liver organogenesis by transactivating a family of greater than 15 predominantly hepatic genes. We have isolated cDNA clones encoding mouse HNF-1 and expressed them in monkey COS cells and in the human T-cell line Jurkat, producing HNF-1 DNA-binding activity as well as transactivation of reporter constructs containing multimerized HNF-1 binding sites. In addition, the HNF-1 gene was assigned by somatic cell hybrids and recombinant inbred strain mapping to mouse chromosome 5 near Bcd-1 and to human chromosome 12 region q22-qter, revealing a homologous chromosome region in these two species. The presence of HNF-1 mRNA in multiple endodermal tissues (liver, stomach, intestine) suggests that HNF-1 may constitute an early marker for endodermal, rather than hepatocyte, differentiation. Further, that HNF-1 DNA-binding and transcriptional activity can be conferred by transfecting the HNF-1 cDNA into several cell lines indicates that it is sufficient to activate transcription in the context of ubiquitously expressed factors.

SUBMITTER: Kuo CJ 

PROVIDER: S-EPMC55269 | biostudies-other | 1990 Dec

REPOSITORIES: biostudies-other

altmetric image

Publications

Molecular cloning, functional expression, and chromosomal localization of mouse hepatocyte nuclear factor 1.

Kuo C J CJ   Conley P B PB   Hsieh C L CL   Francke U U   Crabtree G R GR  

Proceedings of the National Academy of Sciences of the United States of America 19901201 24


The homeodomain-containing transcription factor hepatocyte nuclear factor 1 (HNF-1) most likely plays an essential role during liver organogenesis by transactivating a family of greater than 15 predominantly hepatic genes. We have isolated cDNA clones encoding mouse HNF-1 and expressed them in monkey COS cells and in the human T-cell line Jurkat, producing HNF-1 DNA-binding activity as well as transactivation of reporter constructs containing multimerized HNF-1 binding sites. In addition, the HN  ...[more]

Similar Datasets

| S-EPMC6719700 | biostudies-literature
| S-EPMC1136070 | biostudies-other
| S-EPMC27764 | biostudies-literature
| S-EPMC2939928 | biostudies-literature
| S-EPMC46124 | biostudies-other
| S-EPMC294097 | biostudies-other
| S-EPMC48467 | biostudies-other
| S-EPMC99906 | biostudies-literature
| S-EPMC16364 | biostudies-literature
| S-EPMC1133900 | biostudies-literature