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Determination of cyanide in urine and saliva samples by ion chromatography with pulsed amperometric detection.

ABSTRACT: Commonly known as a highly toxic chemical, cyanide is also an essential reagent for many industrial processes. It naturally occurs in plant seeds as cyanogenic glycosides. Another relatively common mode of cyanide exposure is inhalation of environmental tobacco smoke. This study concerns importance to determine cyanide ion in human biological samples. Urine and saliva samples were collected healthy volunteers exposed to tobacco smoke (active smokers) and environmental tobacco smoke (passive smokers). Chromatographic separation was achieved with an anion-exchange column and separated ions were detected by a pulsed amperometric detector. The method produced linear response in a specific concentration range of cyanide ion. The limit of detection was estimated at 0.1 and 0.5 µg/dm3 for urine and saliva samples, respectively. Cyanide ion concentrations in samples ranged from not detected (below LOD) to 12.88 µg/dm3. The comparison of results of biological samples analyses shows an increasing trend in cyanide concentration that may suggest that environmental tobacco smoke might have an impact on human health.

SUBMITTER: Jaszczak E

PROVIDER: S-EPMC5541122 | biostudies-other | 2017

REPOSITORIES: biostudies-other

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Project description:BackgroundCurrent detection or screening for malaria infection necessitates drawing blood by fingerprick or venipuncture, which poses risks and limitations for repeated measurement. This study presents PCR detection of Plasmodium falciparum in human urine and saliva samples, and illustrates this potential application in genotyping malaria infections.MethodsUrine and saliva were obtained from 47 thick film positive and 4 negative individuals one day after collection of blood slides and filter paper blood spots. P. falciparum DNA was extracted from blood, urine and saliva, in separate groups, using the Chelex method or Qiagen DNEasy kit (urine and saliva only). Blood, urine and saliva extracts were subjected to PCR in separate batches. Amplicons from the various sample types were examined for MSP2 polymorphisms and restriction fragment patterns on DHFR amino acid codon 59.Results and discussionMalaria infections exhibited primarily low-grade parasite densities, with a geometric mean of 775 asexual parasites/mul. Regularly matching polymorphic MSP2 genotypes were found between the corresponding urine, saliva and peripheral blood amplicons of each individual, with different inter-individual polymorphic genotypes. Amplicon yields were significantly dependent on DNA extraction method, parasite density and primer set (p < 0.001). A Qiagen kit extraction had more than 2x higher amplicon yield than the Chelex method, for both urine and saliva. Amplicon yields were 1.6 fold higher from saliva than urine. For each unit increase in log parasite density, the probability of amplicon enhanced 1.8 fold. Highest amplicon yields were obtained from the primer set with the shortest PCR product.ConclusionP. falciparum infection is detectable by PCR on human urine and saliva samples. Subject to further refinement of extraction technique and amplicon yields, large-scale malaria parasite screening and epidemiological surveys could be possible without the need to collect blood and use of needles or sharps.

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Determination of cyanide in urine and saliva samples by ion chromatography with pulsed amperometric detection.

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