Ontology highlight
ABSTRACT:
SUBMITTER: van Eijl RAPM
PROVIDER: S-EPMC5544689 | biostudies-other | 2017 Aug
REPOSITORIES: biostudies-other
van Eijl Roderick A P M RAPM van den Brand Teun T Nguyen Luan N LN Mulder Klaas W KW
Scientific reports 20170804 1
In this study, we originally aimed to characterize the potential role of Argonaute 2 (AGO2) in the nucleus, a key protein of the miRNA machinery. We combined Chromatin Immunoprecipitation (ChIP) with high throughput sequencing (ChIP-seq) and quantitative mass spectrometry (ChIP-MS) using the broadly used AGO2 11A9 antibody to determine interactions with chromatin and nuclear proteins. We found a previously described interaction between AGO2 and SWI/SNF on chromatin with ChIP-MS and observed enri ...[more]