Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo.

ABSTRACT: Mucin-type O-glycosylation occurring in the Golgi apparatus is an important protein posttranslational modification initiated by up to 20 GalNAc-transferase isozymes with largely distinct substrate specificities. Regulation of this enzyme family affects a vast array of proteins transiting the secretory pathway and misregulation causes human diseases. Here we describe the use of protein-based fluorescence sensors that traffic in the secretory pathway to monitor GalNAc-transferase activity in living cells. The sensors can either be "pan" or isozyme specific.

SUBMITTER: Song L 

PROVIDER: S-EPMC5549557 | biostudies-other | 2016

REPOSITORIES: biostudies-other