Ontology highlight
ABSTRACT:
SUBMITTER: Hyland KA
PROVIDER: S-EPMC5549801 | biostudies-other | 2017 Jul
REPOSITORIES: biostudies-other
Hyland Kendra A KA Aronovich Elena L EL Olson Erik R ER Bell Jason B JB Rusten Myra Urness MU Gunther Roland R Hunter David W DW Hackett Perry B PB McIvor R Scott RS
Human gene therapy 20170419 7
The Sleeping Beauty transposon system has been extensively tested for integration of reporter and therapeutic genes in vitro and in vivo in mice. Dogs were used as a large animal model for human therapy and minimally invasive infusion of DNA solutions. DNA solutions were delivered into the entire liver or the left side of the liver using balloon catheters for temporary occlusion of venous outflow. A peak intravascular pressure between 80 and 140 mmHg supported sufficient DNA delivery in dog live ...[more]