Unknown

Dataset Information

0

NRIP-seq: a technique to identify RNA targets of an RNA binding protein on a genome-wide scale.


ABSTRACT: Native RNA immunoprecipitation (nRIP) coupled with high-throughput sequencing (nRIP-seq) is a powerful technique that allows transcriptome-wide identification of the entire subset of coding and noncoding RNAs associated with a particular protein. Since this technology is carried out in a native condition without cross-linking, nRIP-seq detects RNAs that bind a protein directly or indirectly through a larger RNA-protein complex. Here, we use the interaction between RNA and chromatin modifiers, Polycomb proteins, as an example to describe this method. Using nRIP-seq, we provide a snapshot of Ezh2, a Polycomb component, and RNA interaction in mouse embryonic stem cells.

SUBMITTER: Zhao JC 

PROVIDER: S-EPMC5551484 | biostudies-other | 2015

REPOSITORIES: biostudies-other

Similar Datasets

| S-ECPF-GEOD-40591 | biostudies-other
2023-02-09 | PXD038502 | Pride
| S-EPMC10244410 | biostudies-literature
| S-EPMC3193274 | biostudies-other
| S-EPMC9554269 | biostudies-literature
| S-EPMC1885218 | biostudies-literature
| S-EPMC9321434 | biostudies-literature
| S-EPMC9880488 | biostudies-literature
| S-EPMC2800223 | biostudies-literature
| S-EPMC2532738 | biostudies-literature