Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence.
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ABSTRACT: Senescent cells exhibit dramatic changes in protein post-translational modifications. Here, we describe a method, stable isotope labeling with amino acids in cell culture (SILAC) coupled to liquid chromatography tandem mass spectrometry (LC-MS/MS), to identify changes in the ubiquitinome in cells that have undergone oncogene-induced senescence.
SUBMITTER: Zhu H
PROVIDER: S-EPMC5572468 | biostudies-other | 2017
REPOSITORIES: biostudies-other
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