Dataset Information

1H NMR-Based Metabolomics Study of the Toxicological Effects in Rats Induced by "Renqing Mangjue" Pill, a Traditional Tibetan Medicine.

ABSTRACT: "RenqingMangjue" pill (RMP), as an effective prescription of Traditional Tibetan Medicine (TTM), has been widely used in treating digestive diseases and ulcerative colitis for over a thousand years. In certain classical Tibetan Medicine, heavy metal may add as an active ingredient, but it may cause contamination unintentionally in some cases. Therefore, the toxicity and adverse effects of TTM became to draw public attention. In this study, 48 male Wistar rats were orally administrated with different dosages of RMP once a day for 15 consecutive days, then half of the rats were euthanized on the 15th day and the remaining were euthanized on the 30th day. Plasma, kidney and liver samples were acquired to 1H NMR metabolomics analysis. Histopathology and ICP-MS were applied to support the metabolomics findings. The metabolic signature of plasma from RMP-administrated rats exhibited increasing levels of glucose, betaine, and creatine, together with decreasing levels of lipids, 3-hydroxybutate, pyruvate, citrate, valine, leucine, isoleucine, glutamate, and glutamine. The metabolomics analysis results of liver showed that after RMP administration, the concentrations of valine, leucine, proline, tyrosine, and tryptophan elevated, while glucose, sarcosine and 3-hydroxybutyrate decreased. The levels of metabolites in kidney, such as, leucine, valine, isoleucine and tyrosine, were increased, while taurine, glutamate, and glutamine decreased. The study provides several potential biomarkers for the toxicity mechanism research of RMP and shows that RMP may cause injury in kidney and liver and disturbance of several pathways, such as energy metabolism, oxidative stress, glucose and amino acids metabolism.

SUBMITTER: Xu C

PROVIDER: S-EPMC5591455 | biostudies-other | 2017

REPOSITORIES: biostudies-other

ACCESS DATA

Publications

<sup>1</sup>H NMR-Based Metabolomics Study of the Toxicological Effects in Rats Induced by "Renqing Mangjue" Pill, a Traditional Tibetan Medicine.

Xu Can C Rezeng Caidan C Li Jian J Zhang Lan L Yan Yujing Y Gao Jian J Wang Yingfeng Y Li Zhongfeng Z Chen Jianxin J

Frontiers in pharmacology 20170904

"RenqingMangjue" pill (RMP), as an effective prescription of Traditional Tibetan Medicine (TTM), has been widely used in treating digestive diseases and ulcerative colitis for over a thousand years. In certain classical Tibetan Medicine, heavy metal may add as an active ingredient, but it may cause contamination unintentionally in some cases. Therefore, the toxicity and adverse effects of TTM became to draw public attention. In this study, 48 male Wistar rats were orally administrated with diffe ...[more]

PMID: 28928660

Similar Datasets

Project description:CONTEXT:Tsothel, a traditional Tibetan medicine, is regarded as 'the king of essences'. Nevertheless, tsothel has aroused serious concern regarding its biosafety because its main component is HgS. Unfortunately, toxicological studies on tsothel are scarce. OBJECTIVE:As inorganic mercury has high affinity for the kidney, the present investigation was designed to determine the potential nephrotoxicity and mechanism of tsothel. MATERIALS AND METHODS:Sprague-Dawley rats were orally administered different doses of tsothel (0, 66.70, 33.35 and 16.68?mg/kg) daily for 180?days, followed by the withdrawal of tsothel for 120?days. Then, the related nephrotoxicity was examined by the ICP-MS, ELISA, colorimetric, RT-PCR, HE staining, immunohistochemical staining and flow cytometry methods. RESULTS:Although tsothel administration led to a large accumulation of Hg (794.25?±?464.30?ng/g in the 66.70?mg/kg group, 775.75?±?307.89?ng/g in the 33.35?mg/kg group and 532.60?±?356.77?ng/g in the 16.68?mg/kg group) in the kidney after 120?days of tsothel withdrawal, the blood CREA and BUN, urinary Kim-1, NAG, RBP and ?2-MG, renal SOD, MDA, pathology, proliferation, apoptosis and cell cycle had no significant changes compared with the control group. Additionally, the high GSH content (318.87?±?44.19?nmol/mL in the 33.35?mg/kg group) and the relative expression levels of Kim-1 (1.08?±?0.11 in the 33.35?mg/kg group), MT-1 (1.46?±?0.10 in the 66.70?mg/kg group, 1.61?±?0.19 in the 33.35?mg/kg group and 1.57?±?0.14 in the 16.68?mg/kg group) and GST-Pi (1.76?±?0.89 in the 33.35?mg/kg group) mRNA recovered to normal after tsothel withdrawal. Interestingly, the change trend of GST-Pi gene expression was consistent with the change trend of GSH activity. CONCLUSIONS:Overall, our study shows that tsothel administration did not induce overt nephrotoxicity but did have reversible stress-related effects. These results suggest that tsothel affects stress response mechanisms with the involvement of detoxifying enzyme systems. The formulation method and chemotype could play a role in the reduced toxicity potential of tsothel compared to common mercurials.

Dataset Information

1H NMR-Based Metabolomics Study of the Toxicological Effects in Rats Induced by "Renqing Mangjue" Pill, a Traditional Tibetan Medicine.

Publications

<sup>1</sup>H NMR-Based Metabolomics Study of the Toxicological Effects in Rats Induced by "Renqing Mangjue" Pill, a Traditional Tibetan Medicine.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets