Ontology highlight
ABSTRACT:
SUBMITTER: Zlobin N
PROVIDER: S-EPMC5600361 | biostudies-other | 2016 Mar
REPOSITORIES: biostudies-other
Zlobin Nikolai N Evlakov Konstantin K Alekseev Yakov Y Blagodatskikh Konstantin K Babakov Aleksei A Taranov Vasiliy V
Biochemistry and biophysics reports 20160209
Plant cold shock domain proteins (CSDP) participate in maintenance of plant stress tolerance and in regulating their development. In the present paper we show that two out of three extremophyte plant <i>Eutrema salsugineum</i> proteins EsCSDP1-3, namely EsCSDP1 and EsCSDP3, possess high DNA-melting activity. DNA-melting activity of proteins was evaluated using molecular beacon assay in two ways: by measuring Tm parameter (the temperature at which half of the DNA beacon molecules is fully melted) ...[more]