Dataset Information

Thyroid cancer 1 (C8orf4) shows high expression, no mutation and reduced methylation level in lung cancers, and its expression correlates with ?-catenin and DNMT1 expression and poor prognosis.

ABSTRACT: Thyroid cancer 1 (TC1, C8orf4) plays important roles in tumors. The aim of this study was to examine the protein expression levels, methylation status, and mutational status of TC1 (C8orf4) in lung cancers, and investigate the correlation between TC1, other members of the Wnt signaling pathway, and lung cancer. TC1 expression levels were assessed via immunohistochemical staining in 179 cases of lung cancer. ?-catenin, TCF4, Axin, Disabled-2, Chibby, and DNA methyltransferase-1 (DNMT1) expressions were also examined. Bisulfite sequencing PCR analysis was used to examine the methylation status of the C8orf4 locus, while PCR analysis and direct sequencing were used to determine its mutational status. We found high TC1 expression correlated with poor differentiation, advanced TNM stage, lymphatic metastasis, and poor prognosis in lung cancer patients. TC1 expression also correlated with ?-catenin and DNMT1 expressions. No mutations in C8orf4 were detected. However, methylation levels of C8orf4 in lung cancers were lower than in corresponding normal lung tissues. In conclusion, high TC1 expression is implicated in lung cancer progression and correlates with poor prognosis in lung cancer. Reduced methylation levels might be responsible for the elevated TC1 expression levels. TC1, ?-catenin, and DNMT1 can synergistically activate Wnt/?-catenin signaling in lung cancers.

SUBMITTER: Zheng YW

PROVIDER: S-EPMC5609888 | biostudies-other | 2017 Sep

REPOSITORIES: biostudies-other

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Thyroid cancer 1 (C8orf4) shows high expression, no mutation and reduced methylation level in lung cancers, and its expression correlates with β-catenin and DNMT1 expression and poor prognosis.

Zheng Yi-Wen YW Zhang Li L Wang Yuan Y Chen Song-Yan SY Lei Lei L Tang Na N Yang Da-Lei DL Bai Lin-Lin LL Zhang Xiu-Peng XP Jiang Gui-Yang GY Yang Lian-He LH Xu Hong-Tao HT Li Qing-Chang QC Qiu Xue-Shan XS Wang En-Hua EH

Oncotarget 20170406 38

Thyroid cancer 1 (TC1, C8orf4) plays important roles in tumors. The aim of this study was to examine the protein expression levels, methylation status, and mutational status of <i>TC1</i> (<i>C8orf4</i>) in lung cancers, and investigate the correlation between TC1, other members of the Wnt signaling pathway, and lung cancer. TC1 expression levels were assessed via immunohistochemical staining in 179 cases of lung cancer. β-catenin, TCF4, Axin, Disabled-2, Chibby, and DNA methyltransferase-1 (DNM ...[more]

PMID: 28968956

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Dataset Information

Thyroid cancer 1 (C8orf4) shows high expression, no mutation and reduced methylation level in lung cancers, and its expression correlates with ?-catenin and DNMT1 expression and poor prognosis.

Publications

Thyroid cancer 1 (C8orf4) shows high expression, no mutation and reduced methylation level in lung cancers, and its expression correlates with β-catenin and DNMT1 expression and poor prognosis.

Similar Datasets

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