Ontology highlight
ABSTRACT:
SUBMITTER: Shinohara T
PROVIDER: S-EPMC5639258 | biostudies-other | 2017 Oct
REPOSITORIES: biostudies-other
Shinohara Takashi T Kazuki Kanako K Ogonuki Narumi N Morimoto Hiroko H Matoba Shogo S Hiramatsu Kei K Honma Kazuhisa K Suzuki Teruhiko T Hara Takahiko T Ogura Atsuo A Oshimura Mitsuo M Kanatsu-Shinohara Mito M Kazuki Yasuhiro Y
Stem cell reports 20170921 4
The introduction of megabase-sized large DNA fragments into the germline has been a difficult task. Although microcell-mediated chromosome transfer into mouse embryonic stem cells (ESCs) allows the production of transchromosomic mice, ESCs have unstable karyotypes and germline transmission is unreliable by chimera formation. As spermatogonial stem cells (SSCs) are the only stem cells in the germline, they represent an attractive target for germline modification. Here, we report successful transf ...[more]