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Intracellular Uncaging of cGMP with Blue Light.

ABSTRACT: We have made a new caged cGMP that is photolyzed with blue light. Using our recently developed derivative of 7-diethylaminocourmarin (DEAC) called DEAC450, we synthesized coumarin phosphoester derivatives of cGMP with two negative charges appended to the DEAC450 moiety. DEAC450-cGMP is freely soluble in physiological buffer without the need for any organic cosolvents. With a photolysis quantum yield of 0.18 and an extinction coefficient of 43?000 M-1 cm-1 at 453 nm, DEAC450-cGMP is the most photosensitive caged cGMP made to date. In patch-clamped neurons in acutely isolated brain slices, blue light effectively uncaged cGMP from DEAC450 and facilitated activation of hyperpolarization and cyclic nucleotide gated cation (HCN) channels in cholinergic interneurons. Thus, DEAC450-cGMP has a unique set of optical and chemical properties that make it a useful addition to the optical arsenal available to neurobiologists.

SUBMITTER: Agarwal HK

PROVIDER: S-EPMC5647252 | biostudies-other | 2017 Oct

REPOSITORIES: biostudies-other

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Intracellular Uncaging of cGMP with Blue Light.

Agarwal Hitesh K HK Zhai Shenyu S Surmeier D James DJ Ellis-Davies Graham C R GCR

ACS chemical neuroscience 20170801 10

We have made a new caged cGMP that is photolyzed with blue light. Using our recently developed derivative of 7-diethylaminocourmarin (DEAC) called DEAC450, we synthesized coumarin phosphoester derivatives of cGMP with two negative charges appended to the DEAC450 moiety. DEAC450-cGMP is freely soluble in physiological buffer without the need for any organic cosolvents. With a photolysis quantum yield of 0.18 and an extinction coefficient of 43 000 M<sup>-1</sup> cm<sup>-1</sup> at 453 nm, DEAC450 ...[more]

PMID: 28762726

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