Unknown

Dataset Information

0

Ferritin Heavy Subunit Silencing Blocks the Erythroid Commitment of K562 Cells via miR-150 up-Regulation and GATA-1 Repression.


ABSTRACT: Erythroid differentiation is a complex and multistep process during which an adequate supply of iron for hemoglobinization is required. The role of ferritin heavy subunit, in this process, has been mainly attributed to its capacity to maintain iron in a non-toxic form. We propose a new role for ferritin heavy subunit (FHC) in controlling the erythroid commitment of K562 erythro-myeloid cells. FHC knockdown induces a change in the balance of GATA transcription factors and significantly reduces the expression of a repertoire of erythroid-specific genes, including ?- and ?-globins, as well as CD71 and CD235a surface markers, in the absence of differentiation stimuli. These molecular changes are also reflected at the morphological level. Moreover, the ability of FHC-silenced K562 cells to respond to the erythroid-specific inducer hemin is almost completely abolished. Interestingly, we found that this new role for FHC is largely mediated via regulation of miR-150, one of the main microRNA implicated in the cell-fate choice of common erythroid/megakaryocytic progenitors. These findings shed further insight into the biological properties of FHCand delineate a role in erythroid differentiation where this protein does not act as a mere iron metabolism-related factor but also as a critical regulator of the expression of genes of central relevance for erythropoiesis.

SUBMITTER: Zolea F 

PROVIDER: S-EPMC5666848 | biostudies-other | 2017 Oct

REPOSITORIES: biostudies-other

altmetric image

Publications

Ferritin Heavy Subunit Silencing Blocks the Erythroid Commitment of K562 Cells via miR-150 up-Regulation and GATA-1 Repression.

Zolea Fabiana F   Battaglia Anna Martina AM   Chiarella Emanuela E   Malanga Donatella D   De Marco Carmela C   Bond Heather Mandy HM   Morrone Giovanni G   Costanzo Francesco F   Biamonte Flavia F  

International journal of molecular sciences 20171017 10


Erythroid differentiation is a complex and multistep process during which an adequate supply of iron for hemoglobinization is required. The role of ferritin heavy subunit, in this process, has been mainly attributed to its capacity to maintain iron in a non-toxic form. We propose a new role for ferritin heavy subunit (FHC) in controlling the erythroid commitment of K562 erythro-myeloid cells. FHC knockdown induces a change in the balance of GATA transcription factors and significantly reduces th  ...[more]

Similar Datasets

| S-EPMC1221840 | biostudies-other
| S-EPMC4482369 | biostudies-literature
| S-EPMC4767489 | biostudies-literature
| S-EPMC4376865 | biostudies-literature
2017-08-15 | GSE92703 | GEO
| S-EPMC3019278 | biostudies-literature
| S-EPMC5658338 | biostudies-literature
| S-EPMC1890840 | biostudies-literature
| S-EPMC4170028 | biostudies-literature
| S-EPMC6062335 | biostudies-literature