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How gluttonous cell aggregates clear substrates coated with microparticles.

ABSTRACT: We study the spreading of cell aggregates deposited on adhesive substrates decorated with microparticles (MPs). A cell monolayer expands around the aggregate. The cells on the periphery of the monolayer take up the MPs, clearing the substrate as they progress and forming an aureole of cells filled with MPs. We study the dynamics of spreading and determine the width of the aureole and the level of MP internalization in cells as a function of MP size, composition, and density. From the radius and width of the aureole, we quantify the volume fraction of MPs within the cell, which leads to an easy, fast, and inexpensive measurement of the cell - particle internalization.

SUBMITTER: Beaune G

PROVIDER: S-EPMC5691206 | biostudies-other | 2017 Nov

REPOSITORIES: biostudies-other

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How gluttonous cell aggregates clear substrates coated with microparticles.

Beaune Grégory G Lam Andy Y W AYW Dufour Sylvie S Winnik Françoise M FM Brochard-Wyart Françoise F

Scientific reports 20171116 1

We study the spreading of cell aggregates deposited on adhesive substrates decorated with microparticles (MPs). A cell monolayer expands around the aggregate. The cells on the periphery of the monolayer take up the MPs, clearing the substrate as they progress and forming an aureole of cells filled with MPs. We study the dynamics of spreading and determine the width of the aureole and the level of MP internalization in cells as a function of MP size, composition, and density. From the radius and ...[more]

PMID: 29146990

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