Unknown

Dataset Information

0

Bactofilin-mediated organization of the ParABS chromosome segregation system in Myxococcus xanthus.


ABSTRACT: In bacteria, homologs of actin, tubulin, and intermediate filament proteins often act in concert with bacteria-specific scaffolding proteins to ensure the proper arrangement of cellular components. Among the bacteria-specific factors are the bactofilins, a widespread family of polymer-forming proteins whose biology is poorly investigated. Here, we study the three bactofilins BacNOP in the rod-shaped bacterium Myxococcus xanthus. We show that BacNOP co-assemble into elongated scaffolds that restrain the ParABS chromosome segregation machinery to the subpolar regions of the cell. The centromere (parS)-binding protein ParB associates with the pole-distal ends of these structures, whereas the DNA partitioning ATPase ParA binds along their entire length, using the newly identified protein PadC (MXAN_4634) as an adapter. The integrity of these complexes is critical for proper nucleoid morphology and chromosome segregation. BacNOP thus mediate a previously unknown mechanism of subcellular organization that recruits proteins to defined sites within the cytoplasm, far off the cell poles.

SUBMITTER: Lin L 

PROVIDER: S-EPMC5703909 | biostudies-other | 2017 Nov

REPOSITORIES: biostudies-other

altmetric image

Publications

Bactofilin-mediated organization of the ParABS chromosome segregation system in Myxococcus xanthus.

Lin Lin L   Osorio Valeriano Manuel M   Harms Andrea A   Søgaard-Andersen Lotte L   Thanbichler Martin M  

Nature communications 20171128 1


In bacteria, homologs of actin, tubulin, and intermediate filament proteins often act in concert with bacteria-specific scaffolding proteins to ensure the proper arrangement of cellular components. Among the bacteria-specific factors are the bactofilins, a widespread family of polymer-forming proteins whose biology is poorly investigated. Here, we study the three bactofilins BacNOP in the rod-shaped bacterium Myxococcus xanthus. We show that BacNOP co-assemble into elongated scaffolds that restr  ...[more]

Similar Datasets

| S-EPMC7156744 | biostudies-literature
| S-EPMC3778016 | biostudies-literature
| S-EPMC4706359 | biostudies-literature
| S-EPMC3302451 | biostudies-literature
| S-EPMC2579389 | biostudies-literature
| S-EPMC1360536 | biostudies-literature
| S-EPMC3133062 | biostudies-literature
| PRJNA110133 | ENA
| PRJNA120055 | ENA
| PRJNA119265 | ENA