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Flow cytometric discrimination of seven lineage markers by using two fluorochromes.

ABSTRACT: Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.

SUBMITTER: Boin F 

PROVIDER: S-EPMC5708788 | biostudies-other | 2017

REPOSITORIES: biostudies-other

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Flow cytometric discrimination of seven lineage markers by using two fluorochromes.

Boin Francesco F   Giardino Torchia Maria Letizia ML   Borrello Ivan I   Noonan Kimberly A KA   Neil Matthew M   Soloski Mark J MJ   Cimbro Raffaello R  

PloS one 20171130 11

Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping. ...[more]