Dataset Information

High-speed, high-frequency ultrasound, in utero vector-flow imaging of mouse embryos.

ABSTRACT: Real-time imaging of the embryonic murine cardiovascular system is challenging due to the small size of the mouse embryo and rapid heart rate. High-frequency, linear-array ultrasound systems designed for small-animal imaging provide high-frame-rate and Doppler modes but are limited in regards to the field of view that can be imaged at fine-temporal and -spatial resolution. Here, a plane-wave imaging method was used to obtain high-speed image data from in utero mouse embryos and multi-angle, vector-flow algorithms were applied to the data to provide information on blood flow patterns in major organs. An 18-MHz linear array was used to acquire plane-wave data at absolute frame rates ?10?kHz using a set of fixed transmission angles. After beamforming, vector-flow processing and image compounding, effective frame rates were on the order of 2?kHz. Data were acquired from the embryonic liver, heart and umbilical cord. Vector-flow results clearly revealed the complex nature of blood-flow patterns in the embryo with fine-temporal and -spatial resolution.

SUBMITTER: Ketterling JA

PROVIDER: S-EPMC5709407 | biostudies-other | 2017 Nov

REPOSITORIES: biostudies-other

ACCESS DATA

Publications

High-speed, high-frequency ultrasound, in utero vector-flow imaging of mouse embryos.

Ketterling Jeffrey A JA Aristizábal Orlando O Yiu Billy Y S BYS Turnbull Daniel H DH Phoon Colin K L CKL Yu Alfred C H ACH Silverman Ronald H RH

Scientific reports 20171130 1

Real-time imaging of the embryonic murine cardiovascular system is challenging due to the small size of the mouse embryo and rapid heart rate. High-frequency, linear-array ultrasound systems designed for small-animal imaging provide high-frame-rate and Doppler modes but are limited in regards to the field of view that can be imaged at fine-temporal and -spatial resolution. Here, a plane-wave imaging method was used to obtain high-speed image data from in utero mouse embryos and multi-angle, vect ...[more]

PMID: 29192281

Similar Datasets

Project description:PurposeFlow instability has been shown to contribute to the risk of future cardiovascular and cerebrovascular events. Nonetheless, it is challenging to noninvasively detect and identify flow instability in blood vessels. Here, we present a new framework called Doppler ultrasound bandwidth imaging (DUBI) that uses high-frame-rate ultrasound and Doppler bandwidth analysis principles to assess flow instability within an image view.MethodsDoppler ultrasound bandwidth imaging seeks to estimate the instantaneous Doppler bandwidth based on autoregressive modeling at every pixel position of data frames acquired from high-frame-rate plane wave pulsing. This new framework is founded upon the principle that flow instability naturally gives rise to a wide range of flow velocities over a sample volume, and such velocity range in turn yields a larger Doppler bandwidth estimate. The ability for DUBI to map unstable flow was first tested over a range of fluid flow conditions (ranging from laminar to turbulent) with a nozzle-flow phantom. As a further demonstration, DUBI was applied to assess flow instability in healthy and stenosed carotid bifurcation phantoms.ResultsNozzle-flow phantom results showed that DUBI can effectively detect and visualize the difference in Doppler bandwidth magnitude (increased from 2.1 to 5.2 kHz) at stable and unstable flow regions in an image view. Receiver operating characteristic analysis also showed that DUBI can achieve optimal sensitivity and specificity of 0.72 and 0.83, respectively. In the carotid phantom experiments, differences were observed in the spatiotemporal dynamics of Doppler bandwidth over a cardiac cycle. Specifically, as the degree of stenosis increased (from 50% to 75%), DUBI showed an increase in Doppler bandwidth magnitude from 1.4 kHz in the healthy bifurcation to 7.7 kHz at the jet tail located downstream from a 75% stenosis site, thereby indicating flow perturbation in the stenosed bifurcations.ConclusionDUBI can detect unstable flow. This new technique can provide useful hemodynamic information that may aid clinical diagnosis of atherosclerosis.

Dataset Information

High-speed, high-frequency ultrasound, in utero vector-flow imaging of mouse embryos.

Publications

High-speed, high-frequency ultrasound, in utero vector-flow imaging of mouse embryos.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets