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Association of salivary RANKL and osteoprotegerin levels with periodontal health.

ABSTRACT: The aim of this study is to determine the association of salivary levels of the bone remodelling regulators (RANKL) and osteoprotegerin (OPG) with periodontal clinical status. One-hundred fifty-eight participants selected from a pool of adult patients via systematic random sampling were included in this descriptive cross-sectional study carried out at the University of Nairobi Dental Hospital. About 5 ml of unstimulated whole saliva was collected from each participant followed by periodontal clinical examination. Salivary levels of RANKL and OPG were determined using human RANKL and OPG ELISA kits from R&D systems, UK. The mean salivary level of RANKL was 14.65pg/ml (+18.72 SD) with strong association to periodontal disease severity (F = 64.82, p < .001). Mean OPG levels was 139.03 pg/ml (+51.19 SD) with significantly high levels in cases without periodontitis (F = 19.031, p < .001). Consequently, a relative RANKL/OPG ratio was established with a strong positive correlation to disease severity (rs = .759, p < .001). Statistically significant area under curve value of .932 was reported (.1613 cutoff ratio, 95% sensitivity, 6.2% specificity). The levels of RANKL and OPG in saliva and their relative ratio have strong association with the severity of periodontal disease, hence a potential adjunctive diagnostic in evaluating periodontal disease.

SUBMITTER: Ochanji AA

PROVIDER: S-EPMC5719813 | biostudies-other | 2017 Apr

REPOSITORIES: biostudies-other

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Association of salivary RANKL and osteoprotegerin levels with periodontal health.

Ochanji A A AA Matu N K NK Mulli T K TK

Clinical and experimental dental research 20170412 2

The aim of this study is to determine the association of salivary levels of the bone remodelling regulators (RANKL) and osteoprotegerin (OPG) with periodontal clinical status. One-hundred fifty-eight participants selected from a pool of adult patients via systematic random sampling were included in this descriptive cross-sectional study carried out at the University of Nairobi Dental Hospital. About 5 ml of unstimulated whole saliva was collected from each participant followed by periodontal cli ...[more]

PMID: 29744178

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Project description:Purpose:The aims of this study were to examine the salivary microbiota in conditions of periodontal health and disease and to explore microbial changes following nonsurgical periodontal treatment. Methods:Non-stimulated saliva samples were collected from 4 periodontally healthy participants at baseline and from 8 patients with chronic periodontitis at baseline and 3 months following nonsurgical periodontal therapy. The V3 and V4 regions of the 16S rRNA gene from the DNA of saliva samples were amplified and sequenced. The salivary microbial compositions of the healthy participants and patients with periodontitis prior to and following nonsurgical treatment of periodontitis were compared based on the relative abundance of various taxa. Results:On average, 299 operational taxonomic units were identified in each sample. The phylogenetic diversity in patients with periodontitis was higher than that in healthy participants and decreased following treatment. The abundance of the phylum Spirochaetes and the genus Treponema in patients with periodontitis was 143- and 134-fold higher than in the healthy control group, respectively, but decreased significantly following treatment. The species that were overabundant in the saliva of patients with periodontitis included the Peptostreptococcus stomatis group, Porphyromonas gingivalis, the Fusobacterium nucleatum group, Parvimonas micra, Porphyromonas endodontalis, Filifactor alocis, and Tannerella forsythia. The phylum Actinobacteria, the genus Streptococcaceae_uc, and the species Streptococcus salivarius group were more abundant in healthy participants than in those with periodontitis. There was a trend toward a decrease in disease-associated taxa and an increase in health-associated taxa following treatment. Conclusions:Our results revealed differences in the taxa of salivary microbiota between conditions of periodontal health and disease. The taxa found to be associated with health or disease have potential for use as salivary biomarkers for periodontal health or disease.

Project description:IntroductionThe objective of the present study was to evaluate the capacity of a tissue-engineered complex of human osteoprotegerin (hOPG)-transfected periodontal ligament stem cells (PDLSCs) seeding on beta-tricalcium phosphate (β-TCP) to regenerate alveolar bone defects in New Zealand rabbits.MethodsPDLSCs were isolated from rabbit periodontal ligament tissues and expanded in vitro to enrich PDLSC numbers, and their proliferative activities and differentiation capability were evaluated under specific induction conditions. Lentiviral vector containing hOPG and enhanced green fluorescent protein (EGFP) was constructed by using Gateway technology and transfected into rabbit PDLSCs. The expression of hOPG was determined with quantitative real-time reverse transcription-polymerase chain reaction and Western blot. The PDLSCs with or without engineered hOPG were seeded on β-TCP scaffolds prior to transplantation. Morphological characterization of cells and materials was done by scanning electron microscope. Twenty rabbits with alveolar bone defects were randomly allocated into four groups and transplanted with β-TCP, PDLSCs/β-TCP, and hOPG-transfected PDLSCs/β-TCP or were left untreated as a control. Animals were sacrificed 12 weeks after operation for histological observation and histomorphometric analysis.ResultsPDLSCs expressed STRO-1 and vementin and favored osteogenesis and adipogenesis in conditioned media. Expressions of hOPG were significantly upregulated after transfection of the lentiviral vector into PDLSCs. PDLSCs attached and spread well on β-TCP, and there was no significant difference in growth of PDLSCs on β-TCP between the hOPG transfection group and the non-transfection group. The histological observation and histomorphometric analysis showed that the hOPG-transfected PDLSCs/β-TCP complex exhibited an earlier mineralization and more bone formation inside the scaffold than control, β-TCP, and PDLSCs/β-TCP complexes. Implantation of hOPG-transfected PDLSCs contributed to new bone formation as determined by EGFP gene expression under circularly polarized light microscopy.ConclusionsThe present study demonstrated the feasibility of β-TCP scaffolds for primary PDLSC culture and expression of hOPG gene in vitro and in vivo, and hOPG-transfected PDLSCs could serve as a potential cell source for periodontal bone regeneration, which may shed light on the potential of systemic hOPG gene therapy in combination with PDLSC tissue engineering as a good candidate in periodontal tissue engineering for alveolar bone regeneration.

Dataset Information

Association of salivary RANKL and osteoprotegerin levels with periodontal health.

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Association of salivary RANKL and osteoprotegerin levels with periodontal health.

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