Ontology highlight
ABSTRACT:
SUBMITTER: Zhang Z
PROVIDER: S-EPMC5748894 | biostudies-other | 2017 Oct
REPOSITORIES: biostudies-other
Zhang Zhaoyue Z Chen Li L Liu Ling L Su Xiaoyang X Rabinowitz Joshua D JD
Journal of the American Chemical Society 20171004 41
Much understanding of metabolism is based on monitoring chemical reactions in cells with isotope tracers. For this purpose, <sup>13</sup>C is well suited due to its stable incorporation into biomolecules and minimal kinetic isotope effect. For redox reactions, deuterium tracing can provide additional information. To date, studies examining NADPH production with deuterated carbon sources have failed to account for roughly half of NADPH's redox active hydrogen. We show the missing hydrogen is the ...[more]