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High content image analysis reveals function of miR-124 upstream of Vimentin in regulating motor neuron mitochondria.

ABSTRACT: microRNAs (miRNAs) are critical for neuronal function and their dysregulation is repeatedly observed in neurodegenerative diseases. Here, we implemented high content image analysis for investigating the impact of several miRNAs in mouse primary motor neurons. This survey directed our attention to the neuron-specific miR-124, which controls axonal morphology. By performing next generation sequencing analysis and molecular studies, we characterized novel roles for miR-124 in control of mitochondria localization and function. We further demonstrated that the intermediate filament Vimentin is a key target of miR-124 in this system. Our data establishes a new pathway for control of mitochondria function in motor neurons, revealing the value of a neuron-specific miRNA gene as a mechanism for the re-shaping of otherwise ubiquitously-expressed intermediate filament network, upstream of mitochondria activity and cellular metabolism.

SUBMITTER: Yardeni T

PROVIDER: S-EPMC5758812 | biostudies-other | 2018 Jan

REPOSITORIES: biostudies-other

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High content image analysis reveals function of miR-124 upstream of Vimentin in regulating motor neuron mitochondria.

Yardeni Tal T Fine Raquel R Joshi Yuvraj Y Gradus-Pery Tal T Kozer Noga N Reichenstein Irit I Yanowski Eran E Nevo Shir S Weiss-Tishler Hila H Eisenberg-Bord Michal M Shalit Tal T Plotnikov Alexander A Barr Haim M HM Perlson Eran E Hornstein Eran E

Scientific reports 20180108 1

microRNAs (miRNAs) are critical for neuronal function and their dysregulation is repeatedly observed in neurodegenerative diseases. Here, we implemented high content image analysis for investigating the impact of several miRNAs in mouse primary motor neurons. This survey directed our attention to the neuron-specific miR-124, which controls axonal morphology. By performing next generation sequencing analysis and molecular studies, we characterized novel roles for miR-124 in control of mitochondri ...[more]

PMID: 29311649

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High content image analysis reveals function of miR-124 upstream of Vimentin in regulating motor neuron mitochondria.

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High content image analysis reveals function of miR-124 upstream of Vimentin in regulating motor neuron mitochondria.

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