Ontology highlight
ABSTRACT:
SUBMITTER: Yang F
PROVIDER: S-EPMC5815087 | biostudies-other | 2018 Feb
REPOSITORIES: biostudies-other
Yang Fang F Lei Yingying Y Zhou Meiling M Yao Qili Q Han Yichao Y Wu Xiang X Zhong Wanshun W Zhu Chenghang C Xu Weize W Tao Ran R Chen Xi X Lin Da D Rahman Khaista K Tyagi Rohit R Habib Zeshan Z Xiao Shaobo S Wang Dang D Yu Yang Y Chen Huanchun H Fu Zhenfang Z Cao Gang G
Nucleic acids research 20180201 3
Protein-protein interaction (PPI) network maintains proper function of all organisms. Simple high-throughput technologies are desperately needed to delineate the landscape of PPI networks. While recent state-of-the-art yeast two-hybrid (Y2H) systems improved screening efficiency, either individual colony isolation, library preparation arrays, gene barcoding or massive sequencing are still required. Here, we developed a recombination-based 'library vs library' Y2H system (RLL-Y2H), by which multi ...[more]