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A Salt Overly Sensitive Pathway Member from Brassica juncea BjSOS3 Can Functionally Complement ?Atsos3 in Arabidopsis.

ABSTRACT: Salt Overly Sensitive (SOS) pathway is a well-known pathway in arabidopsis, essential for maintenance of ion homeostasis and thus conferring salt stress tolerance. In arabidopsis, the Ca2+ activated SOS3 interacts with SOS2 which further activates SOS1, a Na+/H+ antiporter, responsible for removing toxic sodium ions from the cells. In the present study, we have shown that these three components of SOS pathway, BjSOS1, BjSOS2 and BjSOS3 genes exhibit differential expression pattern in response to salinity and ABA stress in contrasting cultivars of Brassica. It is also noticed that constitutive expression of all the three SOS genes is higher in the tolerant cultivar B. juncea as compared to the sensitive B. nigra. In silico interaction of BjSOS2 and BjSOS3 has been reported recently and here we demonstrate in vivo interaction of these two proteins in onion epidermal peel cells. Further, overexpression of BjSOS3 in corresponding arabidopsis mutant ?Atsos3 was able to rescue the mutant phenotype and exhibit higher tolerance towards salinity stress at the seedling stage.Taken together, these findings demonstrate that the B. juncea SOS3 (BjSOS3) protein is a functional ortholog of its arabidopsis counterpart and thus show a strong functional conservation of SOS pathway responsible for salt stress signalling between arabidopsis and Brassica species.

SUBMITTER: Nutan KK

PROVIDER: S-EPMC5817878 | biostudies-other | 2018 Jan

REPOSITORIES: biostudies-other

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A Salt Overly Sensitive Pathway Member from <i>Brassica juncea</i> BjSOS3 Can Functionally Complement <i>ΔAtsos3</i> in Arabidopsis.

Nutan Kamlesh Kant KK Kumar Gautam G Singla-Pareek Sneh Lata SL Pareek Ashwani A

Current genomics 20180101 1

<h4>Background</h4>Salt Overly Sensitive (SOS) pathway is a well-known pathway in arabidopsis, essential for maintenance of ion homeostasis and thus conferring salt stress tolerance. In arabidopsis, the Ca2+ activated SOS3 interacts with SOS2 which further activates SOS1, a Na+/H+ antiporter, responsible for removing toxic sodium ions from the cells. In the present study, we have shown that these three components of SOS pathway, BjSOS1, BjSOS2 and BjSOS3 genes exhibit differential expression pat ...[more]

PMID: 29491733

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Project description:BackgroundExtensive mapping efforts are currently underway for the establishment of comparative genomics between the model plant, Arabidopsis thaliana and various Brassica species. Most of these studies have deployed RFLP markers, the use of which is a laborious and time-consuming process. We therefore tested the efficacy of PCR-based Intron Polymorphism (IP) markers to analyze genome-wide synteny between the oilseed crop, Brassica juncea (AABB genome) and A. thaliana and analyzed the arrangement of 24 (previously described) genomic block segments in the A, B and C Brassica genomes to study the evolutionary events contributing to karyotype variations in the three diploid Brassica genomes.ResultsIP markers were highly efficient and generated easily discernable polymorphisms on agarose gels. Comparative analysis of the segmental organization of the A and B genomes of B. juncea (present study) with the A and B genomes of B. napus and B. nigra respectively (described earlier), revealed a high degree of colinearity suggesting minimal macro-level changes after polyploidization. The ancestral block arrangements that remained unaltered during evolution and the karyotype rearrangements that originated in the Oleracea lineage after its divergence from Rapa lineage were identified. Genomic rearrangements leading to the gain or loss of one chromosome each between the A-B and A-C lineages were deciphered. Complete homoeology in terms of block organization was found between three linkage groups (LG) each for the A-B and A-C genomes. Based on the homoeology shared between the A, B and C genomes, a new nomenclature for the B genome LGs was assigned to establish uniformity in the international Brassica LG nomenclature code.ConclusionIP markers were highly effective in generating comparative relationships between Arabidopsis and various Brassica species. Comparative genomics between the three Brassica lineages established the major rearrangements, translocations and fusions pivotal to karyotype diversification between the A, B and C genomes of Brassica species. The inter-relationships established between the Brassica lineages vis-à-vis Arabidopsis would facilitate the identification and isolation of candidate genes contributing to traits of agronomic value in crop Brassicas and the development of unified tools for Brassica genomics.

Dataset Information

A Salt Overly Sensitive Pathway Member from Brassica juncea BjSOS3 Can Functionally Complement ?Atsos3 in Arabidopsis.

Publications

A Salt Overly Sensitive Pathway Member from <i>Brassica juncea</i> BjSOS3 Can Functionally Complement <i>ΔAtsos3</i> in Arabidopsis.

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