Dataset Information

BAFF augments IgA2 and IL-10 production by TLR7/8 stimulated total peripheral blood B cells.

ABSTRACT: Class-switching of B cells to IgA can be induced via both T-cell-dependent and T-cell-independent mechanisms. IgA is most predominantly produced mucosally and is important for combating infections and allergies. In contrast to mice, humans have two forms of IgA; IgA1 and IgA2 with diverse tissue distribution. In early life, IgA levels might be sub-optimal especially during the fall season when bacterial and viral infections are more common. Therefore, we investigated using human B cells whether T-cell-independent factors -promoting cell survival, class switching and immunoglobulin secretion- BAFF, APRIL, IL-10 and retinoic acid can boost IgA production in the context of viral or bacterial infection. To this end total and naive peripheral blood B cells were stimulated with these factors for 6 days in the presence or absence of TLR7/8 agonist R848 (mimicking viral infection) or TLR9 agonist CpG-ODN (mimicking bacterial infection). We show that BAFF significantly augments IgA2 production in TLR7/8 stimulated mature, but not naïve B cells. In addition, BAFF augments IL-10 production and viability in TLR7/8 and TLR9 stimulated mature B cells. These data warrant further investigation of its role in immune regulation both in the periphery and mucosal tissues in early life or during disease.

SUBMITTER: den Hartog G

PROVIDER: S-EPMC5836859 | biostudies-other | 2018 Feb

REPOSITORIES: biostudies-other

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BAFF augments IgA2 and IL-10 production by TLR7/8 stimulated total peripheral blood B cells.

den Hartog Gerco G van Osch Thijs L J TLJ Vos Martijn M Meijer Ben B Savelkoul Huub F J HFJ van Neerven R J Joost RJJ Brugman Sylvia S

European journal of immunology 20171011 2

Class-switching of B cells to IgA can be induced via both T-cell-dependent and T-cell-independent mechanisms. IgA is most predominantly produced mucosally and is important for combating infections and allergies. In contrast to mice, humans have two forms of IgA; IgA1 and IgA2 with diverse tissue distribution. In early life, IgA levels might be sub-optimal especially during the fall season when bacterial and viral infections are more common. Therefore, we investigated using human B cells whether ...[more]

PMID: 28921509

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Dataset Information

BAFF augments IgA2 and IL-10 production by TLR7/8 stimulated total peripheral blood B cells.

Publications

BAFF augments IgA2 and IL-10 production by TLR7/8 stimulated total peripheral blood B cells.

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