Unknown

Dataset Information

0

The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling.


ABSTRACT: The mitotic spindle checkpoint delays anaphase onset in the presence of unattached kinetochores, and efficient checkpoint signaling requires kinetochore localization of the Rod-ZW10-Zwilch (RZZ) complex. In the present study, we show that human Chmp4c, a protein involved in membrane remodeling, localizes to kinetochores in prometaphase but is reduced in chromosomes aligned at the metaphase plate. Chmp4c promotes stable kinetochore-microtubule attachments and is required for proper mitotic progression, faithful chromosome alignment, and segregation. Depletion of Chmp4c diminishes localization of RZZ and Mad1-Mad2 checkpoint proteins to prometaphase kinetochores and impairs mitotic arrest when microtubules are depolymerized by nocodazole. Furthermore, Chmp4c binds to ZW10 through a small C-terminal region, and constitutive Chmp4c kinetochore targeting causes a ZW10-dependent checkpoint metaphase arrest. In addition, Chmp4c spindle functions do not require endosomal sorting complex required for transport-dependent membrane remodeling. These results show that Chmp4c regulates the mitotic spindle checkpoint by promoting localization of the RZZ complex to unattached kinetochores.

SUBMITTER: Petsalaki E 

PROVIDER: S-EPMC5839794 | biostudies-other | 2018 Mar

REPOSITORIES: biostudies-other

altmetric image

Publications

The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling.

Petsalaki Eleni E   Dandoulaki Maria M   Zachos George G  

The Journal of cell biology 20180123 3


The mitotic spindle checkpoint delays anaphase onset in the presence of unattached kinetochores, and efficient checkpoint signaling requires kinetochore localization of the Rod-ZW10-Zwilch (RZZ) complex. In the present study, we show that human Chmp4c, a protein involved in membrane remodeling, localizes to kinetochores in prometaphase but is reduced in chromosomes aligned at the metaphase plate. Chmp4c promotes stable kinetochore-microtubule attachments and is required for proper mitotic progre  ...[more]

Similar Datasets

| S-EPMC9879932 | biostudies-literature
| S-EPMC5119518 | biostudies-literature
| S-EPMC3123354 | biostudies-literature
| S-EPMC3754252 | biostudies-literature
| S-EPMC2916704 | biostudies-literature
| S-EPMC1088361 | biostudies-literature
| S-EPMC5018141 | biostudies-literature
| S-EPMC2172676 | biostudies-literature
| S-EPMC9293949 | biostudies-literature
| S-EPMC1182306 | biostudies-literature