Ontology highlight
ABSTRACT:
SUBMITTER: Blaßle A
PROVIDER: S-EPMC5910415 | biostudies-other | 2018 Apr
REPOSITORIES: biostudies-other
Bläßle Alexander A Soh Gary G Braun Theresa T Mörsdorf David D Preiß Hannes H Jordan Ben M BM Müller Patrick P
Nature communications 20180420 1
Fluorescence Recovery After Photobleaching (FRAP) and inverse FRAP (iFRAP) assays can be used to assess the mobility of fluorescent molecules. These assays measure diffusion by monitoring the return of fluorescence in bleached regions (FRAP), or the dissipation of fluorescence from photoconverted regions (iFRAP). However, current FRAP/iFRAP analysis methods suffer from simplified assumptions about sample geometry, bleaching/photoconversion inhomogeneities, and the underlying reaction-diffusion k ...[more]