Dataset Information

TASI: A software tool for spatial-temporal quantification of tumor spheroid dynamics.

ABSTRACT: Spheroid cultures derived from explanted cancer specimens are an increasingly utilized resource for studying complex biological processes like tumor cell invasion and metastasis, representing an important bridge between the simplicity and practicality of 2-dimensional monolayer cultures and the complexity and realism of in vivo animal models. Temporal imaging of spheroids can capture the dynamics of cell behaviors and microenvironments, and when combined with quantitative image analysis methods, enables deep interrogation of biological mechanisms. This paper presents a comprehensive open-source software framework for Temporal Analysis of Spheroid Imaging (TASI) that allows investigators to objectively characterize spheroid growth and invasion dynamics. TASI performs spatiotemporal segmentation of spheroid cultures, extraction of features describing spheroid morpho-phenotypes, mathematical modeling of spheroid dynamics, and statistical comparisons of experimental conditions. We demonstrate the utility of this tool in an analysis of non-small cell lung cancer spheroids that exhibit variability in metastatic and proliferative behaviors.

SUBMITTER: Hou Y

PROVIDER: S-EPMC5940855 | biostudies-other | 2018 May

REPOSITORIES: biostudies-other

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TASI: A software tool for spatial-temporal quantification of tumor spheroid dynamics.

Hou Yue Y Konen Jessica J Brat Daniel J DJ Marcus Adam I AI Cooper Lee A D LAD

Scientific reports 20180508 1

Spheroid cultures derived from explanted cancer specimens are an increasingly utilized resource for studying complex biological processes like tumor cell invasion and metastasis, representing an important bridge between the simplicity and practicality of 2-dimensional monolayer cultures and the complexity and realism of in vivo animal models. Temporal imaging of spheroids can capture the dynamics of cell behaviors and microenvironments, and when combined with quantitative image analysis methods, ...[more]

PMID: 29739990

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Dataset Information

TASI: A software tool for spatial-temporal quantification of tumor spheroid dynamics.

Publications

TASI: A software tool for spatial-temporal quantification of tumor spheroid dynamics.

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