Dataset Information

Low energy cost for cultured pearl formation in grafted chimeric Pinctada margaritifera.

ABSTRACT: The pearl oyster is one of the rare animal models that support two distinct genomes, through the surgical graft process operated for culture pearl production. This grafted organism is assimilated to a chimera whose physiological functioning remains poorly known. The question of the energy expenditure comparison between chimera and non-chimera animals arises. To answer this question, grafted and non-grafted pearl oysters were evaluated for their energetic needs by the indirect calorimetry method. This method made it possible to measure the energy expenditure based on the respiration rate (RR) measurement, reflecting the basal metabolism. The results showed that the RR values for grafted and non-grafted pearl oysters were not significantly different (p?

SUBMITTER: Le Moullac G

PROVIDER: S-EPMC5951858 | biostudies-other | 2018 May

REPOSITORIES: biostudies-other

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Low energy cost for cultured pearl formation in grafted chimeric Pinctada margaritifera.

Le Moullac Gilles G Soyez Claude C Ky Chin-Long CL

Scientific reports 20180514 1

The pearl oyster is one of the rare animal models that support two distinct genomes, through the surgical graft process operated for culture pearl production. This grafted organism is assimilated to a chimera whose physiological functioning remains poorly known. The question of the energy expenditure comparison between chimera and non-chimera animals arises. To answer this question, grafted and non-grafted pearl oysters were evaluated for their energetic needs by the indirect calorimetry method. ...[more]

PMID: 29760480

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Project description:BACKGROUND:The most critical step in the pearl formation during aquaculture is issued to the proliferation and differentiation of outer epithelial cells of mantle graft into pearl sac. This pearl sac secretes various matrix proteins to produce pearls by a complex physiological process which has not been well-understood yet. Here, we aimed to unravel the genes involved in the development of pearl sac and pearl, and the sequential expression patterns of different shell matrix proteins secreted from the pearl sac during pearl formation by pearl oyster Pinctada fucata using high-throughput transcriptome profiling. RESULTS:Principal component analysis (PCA) showed clearly different gene expression profiles between earlier (before 1?week) and later stages (1?week to 3?months) of grafting. Immune-related genes were highly expressed between 0?h - 24?h (donor dependent) and 48?h - 1 w (host dependent), and in the course of wound healing process pearl sac was developed by two weeks of graft transplantation. Moreover, for the first time, we identified some stem cell marker genes including ABCG2, SOX2, MEF2A, HES1, MET, NRP1, ESR1, STAT6, PAX2, FZD1 and PROM1 that were expressed differentially during the formation of pearl sac. The expression profiling of 192 biomineralization-related genes demonstrated that most of the shell matrix proteins (SMPs) involved in prismatic layer formation were first up-regulated and then gradually down-regulated indicating their involvement in the development of pearl sac and the onset of pearl mineralization. Most of the nacreous layer forming SMPs were up-regulated at 2?weeks after the maturation of pearl sac. Nacrein, MSI7 and shematrin involved in both layer formation were highly expressed during 0?h - 24?h, down-regulated up to 1?week and then up-regulated again after accomplishment of pearl sac formation. CONCLUSIONS:Using an RNA-seq approach we unraveled the expression pattern of the key genes involved in the development of pearl sac and pearl as a result of host immune response after grafting. These findings provide valuable information in understanding the molecular mechanism of pearl formation and immune response in P. fucata.

Dataset Information

Low energy cost for cultured pearl formation in grafted chimeric Pinctada margaritifera.

Publications

Low energy cost for cultured pearl formation in grafted chimeric Pinctada margaritifera.

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