Unknown

Dataset Information

0

High-resolution AFM structure of DNA G-wires in aqueous solution.


ABSTRACT: We investigate the self-assembly of short pieces of the Tetrahymena telomeric DNA sequence d[G4T2G4] in physiologically relevant aqueous solution using atomic force microscopy (AFM). Wire-like structures (G-wires) of 3.0?nm height with well-defined surface periodic features were observed. Analysis of high-resolution AFM images allowed their classification based on the periodicity of these features. A major species is identified with periodic features of 4.3?nm displaying left-handed ridges or zigzag features on the molecular surface. A minor species shows primarily left-handed periodic features of 2.2?nm. In addition to 4.3 and 2.2?nm ridges, background features with periodicity of 0.9?nm are also observed. Using molecular modeling and simulation, we identify a molecular structure that can explain both the periodicity and handedness of the major G-wire species. Our results demonstrate the potential structural diversity of G-wire formation and provide valuable insight into the structure of higher-order intermolecular G-quadruplexes. Our results also demonstrate how AFM can be combined with simulation to gain insight into biomolecular structure.

SUBMITTER: Bose K 

PROVIDER: S-EPMC5958085 | biostudies-other | 2018 May

REPOSITORIES: biostudies-other

altmetric image

Publications

High-resolution AFM structure of DNA G-wires in aqueous solution.

Bose Krishnashish K   Lech Christopher J CJ   Heddi Brahim B   Phan Anh Tuân AT  

Nature communications 20180517 1


We investigate the self-assembly of short pieces of the Tetrahymena telomeric DNA sequence d[G<sub>4</sub>T<sub>2</sub>G<sub>4</sub>] in physiologically relevant aqueous solution using atomic force microscopy (AFM). Wire-like structures (G-wires) of 3.0 nm height with well-defined surface periodic features were observed. Analysis of high-resolution AFM images allowed their classification based on the periodicity of these features. A major species is identified with periodic features of 4.3 nm di  ...[more]

Similar Datasets

| S-EPMC8433840 | biostudies-literature
| S-EPMC8587808 | biostudies-literature
| S-EPMC2211690 | biostudies-literature
2015-04-08 | E-ERAD-349 | biostudies-arrayexpress
| S-EPMC2577754 | biostudies-other
| S-EPMC150200 | biostudies-literature
| S-EPMC3405163 | biostudies-literature
| S-EPMC55214 | biostudies-other
| S-EPMC5304584 | biostudies-literature
| S-EPMC5715023 | biostudies-literature