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DPPC Bilayers in Solutions of High Sucrose Content.

ABSTRACT: The properties of lipid bilayers in sucrose solutions have been intensely scrutinized over recent decades because of the importance of sugars in the field of biopreservation. However, a consensus has not yet been formed on the mechanisms of sugar-lipid interaction. Here, we present a study on the effect of sucrose on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers that combines calorimetry, spectral fluorimetry, and optical microscopy. Intriguingly, our results show a significant decrease in the transition enthalpy but only a minor shift in the transition temperature. Our observations can be quantitatively accounted for by a thermodynamic model that assumes partial delayed melting induced by sucrose adsorption at the membrane interface.

SUBMITTER: Morandi MI 

PROVIDER: S-EPMC5961760 | biostudies-other | 2018 May

REPOSITORIES: biostudies-other

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DPPC Bilayers in Solutions of High Sucrose Content.

Morandi Mattia I MI   Sommer Mathieu M   Kluzek Monika M   Thalmann Fabrice F   Schroder André P AP   Marques Carlos M CM  

Biophysical journal 20180501 9

The properties of lipid bilayers in sucrose solutions have been intensely scrutinized over recent decades because of the importance of sugars in the field of biopreservation. However, a consensus has not yet been formed on the mechanisms of sugar-lipid interaction. Here, we present a study on the effect of sucrose on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers that combines calorimetry, spectral fluorimetry, and optical microscopy. Intriguingly, our results show a significant decrease i  ...[more]