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Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production.

ABSTRACT: Excessive nitric oxide (NO) production by macrophages has been involved in inflammatory diseases. Seven polyphenols (1-7) were isolated from Broussonetia kazinoki (B. kazinoki) and investigated as potential inhibitors of NO overproduction in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Among them, four prenylated polyphenols (2-4 and 6) with a catechol moiety efficiently suppressed the LPS-induced high level of NO with IC50 values of less than 6 µM. The compounds 2-4 and 6 also attenuated protein and mRNA levels of inducible nitric oxide synthase (iNOS). Moreover, they suppressed the nuclear factor ?B (NF-?B) activity by inhibiting the degradation of inhibitory-?B-? (I-?B-?) and the translocation of NF-?B into the nucleus in LPS-activated macrophages. Taken together, these findings suggest that polyphenols from B. kazinoki might be beneficial for treatment of inflammatory diseases.

SUBMITTER: Lee DY

PROVIDER: S-EPMC6017281 | biostudies-other | 2018 Mar

REPOSITORIES: biostudies-other

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Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production.

Lee Da Yeon DY Lee Hwa Jin HJ Ryu Jae-Ha JH

Molecules (Basel, Switzerland) 20180312 3

Excessive nitric oxide (NO) production by macrophages has been involved in inflammatory diseases. Seven polyphenols (1-7) were isolated from Broussonetia kazinoki (B. kazinoki) and investigated as potential inhibitors of NO overproduction in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Among them, four prenylated polyphenols (2-4 and 6) with a catechol moiety efficiently suppressed the LPS-induced high level of NO with IC50 values of le ...[more]

PMID: 29534539

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