Ontology highlight
ABSTRACT:
SUBMITTER: Desai N
PROVIDER: S-EPMC6020757 | biostudies-other | 2016 Aug
REPOSITORIES: biostudies-other
Desai Nikunj N Thomas Daniel A DA Lee Jungeun J Gao Jinshan J Beauchamp J L JL
Chemical science 20160505 8
Mass spectrometric glycan rearrangement is problematic because it provides misleading structural information. Here we report on a new reagent, a methylated free radical activated glycan sequencing reagent (Me-FRAGS), which combines a free radical precursor with a methylated pyridine moiety that can be coupled to the reducing terminus of glycans. The collisional activation of Me-FRAGS-derivatized glycans generates a nascent free radical that concurrently induces abundant glycosidic bond and cross ...[more]