Dataset Information

The Effect of Feeding Cocoa Powder and Lactobacillus rhamnosus on the Composition and Function of Pig Intestinal Microbiome.

ABSTRACT: Dietary habits have been linked with variability of gut microbiota composition and disease risk.The aim of this study was to evaluate the effect of feeding a cocoa powder with or without a probiotic on the composition and function of the fecal microbiome of pigs.Four groups of 8 pigs each were fed a standard growth diet supplemented with cocoa powder, Lactobacillus rhamnosus (LGG), cocoa powder + LGG, or an equal amount of fiber similar to that found in cocoa powder (control group). Fecal samples were collected prior to and 4 wk after initiation of the dietary intervention. Microbiota composition was determined after amplification of the first 2 variable regions of the 16S ribosomal DNA (rDNA). Predictions of metagenomic function were calculated using 16S rDNA sequence data through Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt).After 4 wk of treatment, bacterial abundance analysis demonstrated a prebiotic effect of cocoa powder on endogenous Bifidobacteriaceae and Lactobacillaceae and increased abundance of saccharolytic butyrate-producing bacteria like Roseburia. An increased bacterial evenness, Shannon diversity index, and diverse metabolic profile were detected in microbiomes of pigs fed the cocoa powder + LGG (P < 0.05) but not in pigs in the other 3 groups.The data generated from this work demonstrated that 4-wk dietary treatment with cocoa powder alone or in combination with LGG probiotic had an impact on the composition and function of the fecal microbiota of healthy pigs.

SUBMITTER: Solano-Aguilar GI

PROVIDER: S-EPMC6041806 | biostudies-other | 2018 May

REPOSITORIES: biostudies-other

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The Effect of Feeding Cocoa Powder and <i>Lactobacillus rhamnosus</i> on the Composition and Function of Pig Intestinal Microbiome.

Solano-Aguilar Gloria I GI Lakshman Sukla S Jang Saebyeol S Beshah Ethiopia E Xie Yue Y Sikaroodi Masoumeh M Gupta Richi R Vinyard Bryan B Molokin Aleksey A Urban Joseph F JF Gillevet Patrick P Davis Cindy D CD

Current developments in nutrition 20180209 5

<h4>Background</h4>Dietary habits have been linked with variability of gut microbiota composition and disease risk.<h4>Objective</h4>The aim of this study was to evaluate the effect of feeding a cocoa powder with or without a probiotic on the composition and function of the fecal microbiome of pigs.<h4>Methods</h4>Four groups of 8 pigs each were fed a standard growth diet supplemented with cocoa powder, <i>Lactobacillus rhamnosus</i> (LGG), cocoa powder + LGG, or an equal amount of fiber similar ...[more]

PMID: 30019034

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Dataset Information

The Effect of Feeding Cocoa Powder and Lactobacillus rhamnosus on the Composition and Function of Pig Intestinal Microbiome.

Publications

The Effect of Feeding Cocoa Powder and <i>Lactobacillus rhamnosus</i> on the Composition and Function of Pig Intestinal Microbiome.

Similar Datasets

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