Ontology highlight
ABSTRACT:
SUBMITTER: Sternon JF
PROVIDER: S-EPMC6056880 | biostudies-other | 2018 Aug
REPOSITORIES: biostudies-other
Sternon Jean-François JF Godessart Pierre P Gonçalves de Freitas Rosa R Van der Henst Mathilde M Poncin Katy K Francis Nayla N Willemart Kevin K Christen Matthias M Christen Beat B Letesson Jean-Jacques JJ De Bolle Xavier X
Infection and immunity 20180723 8
<i>Brucella abortus</i> is a class III zoonotic bacterial pathogen able to survive and replicate inside host cells, including macrophages. Here we report a multidimensional transposon sequencing analysis to identify genes essential for <i>Brucella abortus</i> growth in rich medium and replication in RAW 264.7 macrophages. The construction of a dense transposon mutant library and mapping of 929,769 unique mini-Tn<i>5</i> insertion sites in the genome allowed identification of 491 essential coding ...[more]