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Searching for shelter in a ferruginous cave? A new species of Pasipha from a plateau in the Brazilian savanna (Platyhelminthes, Tricladida).

ABSTRACT: In a fauna survey in the eastern margin of Serra do Espinhaço Plateau, in an area belonging to the Brazilian savanna (Cerrado phytophysiognomy), a land flatworm was sampled in a ferruginous cave. Anatomical and histological analyses indicated that it belongs to a new species of the genus Pasipha, which is herein described. The new species shows an almost homogenous dark brown dorsal pigmentation, eyes spreading over the dorsal surface, a collar-shaped pharynx, and a prostatic vesicle with two portions separated by a canal. It differs from similar species mainly by anatomical and histological details of the ejaculatory duct, as well as male and female atria. The flatworm shows no troglomorphic traits and was collected once in the entrance zone of the cave. Hence, despite representing the first land flatworm species described from a Neotropical cave, we consider that its occurrence in the cave is probably occasional, using it as a shelter.

SUBMITTER: Leal-Zanchet A

PROVIDER: S-EPMC6072825 | biostudies-other | 2018

REPOSITORIES: biostudies-other

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Searching for shelter in a ferruginous cave? A new species of <i>Pasipha</i> from a plateau in the Brazilian savanna (Platyhelminthes, Tricladida).

Leal-Zanchet Ana A Marques Alessandro A

ZooKeys 20180726 776

In a fauna survey in the eastern margin of Serra do Espinhaço Plateau, in an area belonging to the Brazilian savanna (Cerrado phytophysiognomy), a land flatworm was sampled in a ferruginous cave. Anatomical and histological analyses indicated that it belongs to a new species of the genus <i>Pasipha</i>, which is herein described. The new species shows an almost homogenous dark brown dorsal pigmentation, eyes spreading over the dorsal surface, a collar-shaped pharynx, and a prostatic vesicle with ...[more]

PMID: 30100782

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Project description:BACKGROUND: The EcoTILLING technique allows polymorphisms in target genes of natural populations to be quickly analysed or identified and facilitates the screening of genebank collections for desired traits. We have developed an EcoTILLING platform to exploit Capsicum genetic resources. A perfect example of the utility of this EcoTILLING platform is its application in searching for new virus-resistant alleles in Capsicum genus. Mutations in translation initiation factors (eIF4E, eIF(iso)4E, eIF4G and eIF(iso)4G) break the cycle of several RNA viruses without affecting the plant life cycle, which makes these genes potential targets to screen for resistant germplasm. RESULTS: We developed and assayed a cDNA-based EcoTILLING platform with 233 cultivated accessions of the genus Capsicum. High variability in the coding sequences of the eIF4E and eIF(iso)4E genes was detected using the cDNA platform. After sequencing, 36 nucleotide changes were detected in the CDS of eIF4E and 26 in eIF(iso)4E. A total of 21 eIF4E haplotypes and 15 eIF(iso)4E haplotypes were identified. To evaluate the functional relevance of this variability, 31 possible eIF4E/eIF(iso)4E combinations were tested against Potato virus Y. The results showed that five new eIF4E variants (pvr2(10), pvr2(11), pvr2(12), pvr2(13) and pvr2(14)) were related to PVY-resistance responses. CONCLUSIONS: EcoTILLING was optimised in different Capsicum species to detect allelic variants of target genes. This work is the first to use cDNA instead of genomic DNA in EcoTILLING. This approach avoids intronic sequence problems and reduces the number of reactions. A high level of polymorphism has been identified for initiation factors, showing the high genetic variability present in our collection and its potential use for other traits, such as genes related to biotic or abiotic stresses, quality or production. Moreover, the new eIF4E and eIF(iso)4E alleles are an excellent collection for searching for new resistance against other RNA viruses.

Dataset Information

Searching for shelter in a ferruginous cave? A new species of Pasipha from a plateau in the Brazilian savanna (Platyhelminthes, Tricladida).

Publications

Searching for shelter in a ferruginous cave? A new species of <i>Pasipha</i> from a plateau in the Brazilian savanna (Platyhelminthes, Tricladida).

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