Unknown

Dataset Information

0

The Protozoan Parasite Toxoplasma gondii Selectively Reprograms the Host Cell Translatome.


ABSTRACT: The intracellular parasite Toxoplasma gondii promotes infection by targeting multiple host cell processes; however, whether it modulates mRNA translation is currently unknown. Here, we show that infection of primary murine macrophages with type I or II T. gondii strains causes a profound perturbation of the host cell translatome. Notably, translation of transcripts encoding proteins involved in metabolic activity and components of the translation machinery was activated upon infection. In contrast, the translational efficiency of mRNAs related to immune cell activation and cytoskeleton/cytoplasm organization was largely suppressed. Mechanistically, T. gondii bolstered mechanistic target of rapamycin (mTOR) signaling to selectively activate the translation of mTOR-sensitive mRNAs, including those with a 5'-terminal oligopyrimidine (5' TOP) motif and those encoding mitochondrion-related proteins. Consistent with parasite modulation of host mTOR-sensitive translation to promote infection, inhibition of mTOR activity suppressed T. gondii replication. Thus, selective reprogramming of host mRNA translation represents an important subversion strategy during T. gondii infection.

SUBMITTER: Leroux LP 

PROVIDER: S-EPMC6105892 | biostudies-other | 2018 Sep

REPOSITORIES: biostudies-other

altmetric image

Publications

The Protozoan Parasite Toxoplasma gondii Selectively Reprograms the Host Cell Translatome.

Leroux Louis-Philippe LP   Lorent Julie J   Graber Tyson E TE   Chaparro Visnu V   Masvidal Laia L   Aguirre Maria M   Fonseca Bruno D BD   van Kempen Léon C LC   Alain Tommy T   Larsson Ola O   Jaramillo Maritza M  

Infection and immunity 20180822 9


The intracellular parasite <i>Toxoplasma gondii</i> promotes infection by targeting multiple host cell processes; however, whether it modulates mRNA translation is currently unknown. Here, we show that infection of primary murine macrophages with type I or II <i>T. gondii</i> strains causes a profound perturbation of the host cell translatome. Notably, translation of transcripts encoding proteins involved in metabolic activity and components of the translation machinery was activated upon infect  ...[more]

Similar Datasets

| S-EPMC1140536 | biostudies-literature
2005-08-13 | GSE3119 | GEO
2005-08-13 | E-GEOD-3119 | biostudies-arrayexpress
| S-EPMC1317489 | biostudies-literature
| S-EPMC2258246 | biostudies-literature
| S-EPMC2168421 | biostudies-literature
| S-EPMC9131557 | biostudies-literature
| S-EPMC10185354 | biostudies-literature
| S-EPMC7509071 | biostudies-literature
| S-EPMC4148248 | biostudies-literature