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Intrinsic refractive index matched 3D dSTORM with two objectives: Comparison of detection techniques.

ABSTRACT: We have built a setup for 3D single molecule localisation microscopy (SMLM) where a very high resolution is achieved by, firstly, the use of two objectives instead of one and, secondly, minimizing optical aberrations by refractive index matching with a glycerol-water mixture as immersion medium in conjunction with glycerol-immersion objectives. Multiple optical paths of the microscope allow to switch between astigmatic and interferometric localisation along the optical axis, thus enabling a direct comparison of the performance of these localisation methods.

SUBMITTER: Schmidt NC

PROVIDER: S-EPMC6127109 | biostudies-other | 2018 Sep

REPOSITORIES: biostudies-other

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Intrinsic refractive index matched 3D dSTORM with two objectives: Comparison of detection techniques.

Schmidt Nora C NC Kahms Martin M Hüve Jana J Klingauf Jürgen J

Scientific reports 20180906 1

We have built a setup for 3D single molecule localisation microscopy (SMLM) where a very high resolution is achieved by, firstly, the use of two objectives instead of one and, secondly, minimizing optical aberrations by refractive index matching with a glycerol-water mixture as immersion medium in conjunction with glycerol-immersion objectives. Multiple optical paths of the microscope allow to switch between astigmatic and interferometric localisation along the optical axis, thus enabling a dire ...[more]

PMID: 30190491

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