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Human adenovirus type 17 from species D transduces endothelial cells and human CD46 is involved in cell entry.

ABSTRACT: More than 70 human adenoviruses with type-dependent pathogenicity have been identified but biological information about the majority of these virus types is scarce. Here we employed multiple sequence alignments and structural information to predict receptor usage for the development of an adenoviral vector with novel biological features. We report the generation of a cloned adenovirus based on human adenovirus type 17 (HAdV17) with high sequence homology to the well characterized human adenovirus type 37 (HAdV37) that causes epidemic keratoconjunctivitis (EKC). Our study revealed that human CD46 (CD46) is involved in cell entry of HAdV17. Moreover, we found that HAdV17 infects endothelial cells (EC) in vitro including primary cells at higher efficiencies compared to the commonly used human adenovirus type 5 (HAdV5). Using a human CD46 transgenic mouse model, we observed that HAdV17 displays a broad tropism in vivo after systemic injection and that it transduces ECs in this mouse model. We conclude that the HAdV17-based vector may provide a novel platform for gene therapy.

SUBMITTER: Liu J

PROVIDER: S-EPMC6128842 | biostudies-other | 2018 Sep

REPOSITORIES: biostudies-other

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Human adenovirus type 17 from species D transduces endothelial cells and human CD46 is involved in cell entry.

Liu Jing J Boehme Philip P Zhang Wenli W Fu Jun J Yumul Roma R Mese Kemal K Tsoukas Raphael R Solanki Manish M Kaufmann Michael M Lu Ruirui R Schmidtko Achim A Stewart A Francis AF Lieber André A Ehrhardt Anja A

Scientific reports 20180907 1

More than 70 human adenoviruses with type-dependent pathogenicity have been identified but biological information about the majority of these virus types is scarce. Here we employed multiple sequence alignments and structural information to predict receptor usage for the development of an adenoviral vector with novel biological features. We report the generation of a cloned adenovirus based on human adenovirus type 17 (HAdV17) with high sequence homology to the well characterized human adenoviru ...[more]

PMID: 30194327

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Human adenovirus type 17 from species D transduces endothelial cells and human CD46 is involved in cell entry.

Publications

Human adenovirus type 17 from species D transduces endothelial cells and human CD46 is involved in cell entry.

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