Ontology highlight
ABSTRACT:
SUBMITTER: Afek A
PROVIDER: S-EPMC6136900 | biostudies-other | 2018 Apr
REPOSITORIES: biostudies-other
Afek Ariel A Ilic Stefan S Horton John J Lukatsky David B DB Gordan Raluca R Akabayov Barak B
iScience 20180327
Primases are key enzymes involved in DNA replication. They act on single-stranded DNA and catalyze the synthesis of short RNA primers used by DNA polymerases. Here, we investigate the DNA binding and activity of the bacteriophage T7 primase using a new workflow called high-throughput primase profiling (HTPP). Using a unique combination of high-throughput binding assays and biochemical analyses, HTPP reveals a complex landscape of binding specificity and functional activity for the T7 primase, de ...[more]