IFN-?R2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis.

ABSTRACT: OBJECTIVE:Chronic periodontitis (CP) is characterized by gingival inflammation and bone destruction. It has been reported that interferon-gamma (IFN-?) levels are high in CP patients; however, the IFN-? receptor (IFN-?R) has not been studied in gingival tissue from these patients. To evaluate IFN-? levels and IFN-?R expression in gingival tissue biopsies from chronic periodontitis patients compared with healthy subjects (HS). MATERIAL AND METHODS:Gingival tissues were obtained from all study subjects, CP (n = 18) and healthy subjects (HS) (n = 12). A tissue section of each study subject was embedded in paraffin blocks to determine the expression of IFN-? R (IFN-?R1 and IFN-?R2) through immunohistochemistry. Another section of the tissue was homogenized and IFN-? was measured by the ELISA technique. RESULTS:No significant differences were found in the IFN-?R1 expression within the cell layers of the gingival tissue of the study groups. When analyzing the IFN-?R2 expression it was found that IFN-?R2 is strongly expressed in the endothelial cells of CP patients when compared to HS (p<0.05). IFN-? concentrations in the gingival tissue were significantly higher in CP patients than in HS. No significant correlation between IFN-? levels and the expression of IFN-?R1 and IFN-?R2 was found. However, a positive correlation between IFN-? levels and clinical parameters [probing depth (PD) and clinical attachment level (CAL)] was found. CONCLUSION:The study of IFN-?R expression in gingival tissue samples from patients with CP showed an increase only in the IFN-?R2 chain in endothelial cells when compared to HS.

SUBMITTER: Franco-Topete R 

PROVIDER: S-EPMC6172018 | biostudies-other | 2018 Oct

REPOSITORIES: biostudies-other