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IFN-?R2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis.

ABSTRACT: OBJECTIVE:Chronic periodontitis (CP) is characterized by gingival inflammation and bone destruction. It has been reported that interferon-gamma (IFN-?) levels are high in CP patients; however, the IFN-? receptor (IFN-?R) has not been studied in gingival tissue from these patients. To evaluate IFN-? levels and IFN-?R expression in gingival tissue biopsies from chronic periodontitis patients compared with healthy subjects (HS). MATERIAL AND METHODS:Gingival tissues were obtained from all study subjects, CP (n = 18) and healthy subjects (HS) (n = 12). A tissue section of each study subject was embedded in paraffin blocks to determine the expression of IFN-? R (IFN-?R1 and IFN-?R2) through immunohistochemistry. Another section of the tissue was homogenized and IFN-? was measured by the ELISA technique. RESULTS:No significant differences were found in the IFN-?R1 expression within the cell layers of the gingival tissue of the study groups. When analyzing the IFN-?R2 expression it was found that IFN-?R2 is strongly expressed in the endothelial cells of CP patients when compared to HS (p<0.05). IFN-? concentrations in the gingival tissue were significantly higher in CP patients than in HS. No significant correlation between IFN-? levels and the expression of IFN-?R1 and IFN-?R2 was found. However, a positive correlation between IFN-? levels and clinical parameters [probing depth (PD) and clinical attachment level (CAL)] was found. CONCLUSION:The study of IFN-?R expression in gingival tissue samples from patients with CP showed an increase only in the IFN-?R2 chain in endothelial cells when compared to HS.

SUBMITTER: Franco-Topete R

PROVIDER: S-EPMC6172018 | biostudies-other | 2018 Oct

REPOSITORIES: biostudies-other

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IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis.

Franco-Topete Ramón R Zepeda-Nuño José Sergio JS Zamora-Perez Ana Lourdes AL Fuentes-Lerma Martha Graciela MG Gómez-Meda Belinda Claudia BC Guerrero-Velázquez Celia C

Journal of applied oral science : revista FOB 20181004

<h4>Objective</h4>Chronic periodontitis (CP) is characterized by gingival inflammation and bone destruction. It has been reported that interferon-gamma (IFN-γ) levels are high in CP patients; however, the IFN-γ receptor (IFN-γR) has not been studied in gingival tissue from these patients. To evaluate IFN-γ levels and IFN-γR expression in gingival tissue biopsies from chronic periodontitis patients compared with healthy subjects (HS).<h4>Material and methods</h4>Gingival tissues were obtained fro ...[more]

PMID: 30304122

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Project description:This study aimed to investigate the relationship between inflammation-related T-helper cell polarization and the receptor activator for nuclear factor-κB ligand (RANKL)/osteoprotegerin (OPG) ratio, which is associated with bone resorption or remodeling of chronic periodontitis patients. Gingival crevicular fluid (GCF) and gingival tissues were obtained from periodontally healthy individuals (PH group) and chronic periodontitis patients (CP group). The GCF levels of IFN-γ, IL-4, IL-17, and IL-10 linked to T-helper cell polarization toward the Th1, Th2, Th17, and Treg phenotypes, respectively, were determined by ELISA. The expression levels of these cytokines and the polarized T-helper cells in gingival tissues were assessed through immunohistochemical and immunofluorescence assays. In addition, the RANKL and OPG expression levels in gingival tissues were detected by immunohistochemical assays, and linear regression analysis was used to identify the potential relationship between T-helper cell polarization and the RANKL/OPG ratio. In total, 22 individuals and 35 patients were enrolled in the present study. In both GCF and gingival tissues, increased levels of IL-17 and the decreased levels of IL-4 and IL-10 were observed in the CP group. When polarized T-helper cells were identified in gingival tissues, more Th1 and Th17 cells were found in the CP group, whereas more Th2 and Treg cells were found in the PH group. Although there was no significant difference in OPG expression between the two groups, the RANKL/OPG ratio in the CP group was higher than that in the PH group. The linear regression analysis showed that the presence of more Th1 and Th17 cells correlated with a higher RANKL/OPG ratio, whereas the presence of more Th2 cells correlated with a lower RANKL/OPG ratio. Th1 and Th17 cells are positively correlated and Th2 cells are negatively correlated with the RANKL/OPG ratio. Our data suggest that T-helper cell polarization is closely linked to the RANKL/OPG ratio in gingival tissues from chronic periodontitis patients.

Project description:Systemic lupus erythematosus (SLE) is a complex chronic autoimmune disease characterized by tissue damage and widespread inflammation in response to environmental challenges. Deposition of immune complexes in kidneys glomeruli are associated with lupus nephritis, determining SLE diagnosis. Periodontitis is a chronic inflammatory disease characterized by clinical attachment and bone loss, caused by a microbial challenge - host response interaction. Deposition of immune complex at gingival tissues is a common finding in the course of the disease. Considering that, the primary aim of this study is to investigate the deposition of immune complexes at gingival tissues of SLE patients compared to systemically healthy ones, correlating it to periodontal and systemic parameters. Twenty-five women diagnosed with SLE (SLE+) and 25 age-matched systemically healthy (SLE-) women were included in the study. Detailed information on overall patient's health were obtained from file records. Participants were screened for probing depth (PD), clinical attachment loss (CAL), gingival recession (REC), full-mouth bleeding score (FMBS) and plaque scores (FMPS). Bone loss was determined at panoramic X-ray images as the distance from cementenamel junction to alveolar crest (CEJ-AC). Gingival biopsies were obtained from the first 15 patients submitted to surgical periodontal therapy of each group, and were analyzed by optical microscopy and direct immunofluorescence to investigate the deposition of antigen-antibody complexes. Eleven (44%) patients were diagnosed with active SLE (SLE-A) and 14 (56%) with inactive SLE (LES-I). Mean PD, CAL and FMBS were significantly lower in SLE+ than SLE-(p < 0.05; Mann Whitney). The chronic use of low doses of immunosuppressants was associated with lower prevalence of CAL >3 mm. Immunofluorescence staining of markers of lupus nephritis and/or proteinuria was significantly increased in SLE+ compared to SLE-, even in the presence of periodontitis. These findings suggest that immunomodulatory drugs in SLE improves periodontal parameters. The greater deposition of antigen-antibody complexes in the gingival tissues of patients diagnosed with SLE may be a marker of disease activity, possibly complementing their diagnosis.

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IFN-?R2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis.

Publications

IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis.

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