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Magnetic Resonance Spectroscopy to Study Glycolytic Metabolism During Autophagy.

ABSTRACT: Cancer cells undergoing starvation- and treatment-induced autophagy were found to exhibit reduced intracellular lactate, reduced rates of steady-state lactate excretion and reduced real-time pyruvate-lactate exchange rates, indicating that glycolytic metabolism was altered in autophagic cells. In this chapter, we describe the technical details of the use of 1H-magnetic resonance spectroscopy (MRS) to measure endogenous cellular concentrations of lactate and glucose in autophagic cells and tissues, how to measure the rate of steady-state lactate excretion and glucose uptake by 1H-MRS in autophagic cells, and details of the real-time measurement of [1-13C] pyruvate to lactate exchange in autophagic cells by 13C-MRS-DNP (dynamic nuclear polarization).

SUBMITTER: Chung YL 

PROVIDER: S-EPMC6175046 | biostudies-other | 2017

REPOSITORIES: biostudies-other

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Magnetic Resonance Spectroscopy to Study Glycolytic Metabolism During Autophagy.

Chung Y-L YL   Leach M O MO   Eykyn T R TR  

Methods in enzymology 20161117

Cancer cells undergoing starvation- and treatment-induced autophagy were found to exhibit reduced intracellular lactate, reduced rates of steady-state lactate excretion and reduced real-time pyruvate-lactate exchange rates, indicating that glycolytic metabolism was altered in autophagic cells. In this chapter, we describe the technical details of the use of <sup>1</sup>H-magnetic resonance spectroscopy (MRS) to measure endogenous cellular concentrations of lactate and glucose in autophagic cells  ...[more]