Magnetic Resonance Spectroscopy to Study Glycolytic Metabolism During Autophagy.
Ontology highlight
ABSTRACT:
SUBMITTER: Chung YL
PROVIDER: S-EPMC6175046 | biostudies-other | 2017
REPOSITORIES: biostudies-other
Publications
Magnetic Resonance Spectroscopy to Study Glycolytic Metabolism During Autophagy.
Chung Y-L YL Leach M O MO Eykyn T R TR
Methods in enzymology 20161117
Cancer cells undergoing starvation- and treatment-induced autophagy were found to exhibit reduced intracellular lactate, reduced rates of steady-state lactate excretion and reduced real-time pyruvate-lactate exchange rates, indicating that glycolytic metabolism was altered in autophagic cells. In this chapter, we describe the technical details of the use of <sup>1</sup>H-magnetic resonance spectroscopy (MRS) to measure endogenous cellular concentrations of lactate and glucose in autophagic cells ...[more]
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