Ontology highlight
ABSTRACT:
SUBMITTER: Li X
PROVIDER: S-EPMC6178885 | biostudies-other | 2018 Oct
REPOSITORIES: biostudies-other
Li Xiang X Tao Yezheng Y Bradley Robert R Du Zhongwei Z Tao Yunlong Y Kong Linghai L Dong Yi Y Jones Jeffrey J Yan Yuanwei Y Harder Cole R K CRK Friedman Lindsay Morgan LM Bilal Magd M Hoffmann Brianna B Zhang Su-Chun SC
Stem cell reports 20180927 4
Differentiation of astrocytes from human pluripotent stem cells (hPSCs) is a tedious and variable process. This hampers the study of hPSC-generated astrocytes in disease processes and drug development. By using CRISPR/Cas9-mediated inducible expression of NFIA or NFIA plus SOX9 in hPSCs, we developed a method to efficiently generate astrocytes in 4-7 weeks. The astrocytic identity of the induced cells was verified by their characteristic molecular and functional properties as well as after trans ...[more]