Unknown

Dataset Information

0

Fast Generation of Functional Subtype Astrocytes from Human Pluripotent Stem Cells.


ABSTRACT: Differentiation of astrocytes from human pluripotent stem cells (hPSCs) is a tedious and variable process. This hampers the study of hPSC-generated astrocytes in disease processes and drug development. By using CRISPR/Cas9-mediated inducible expression of NFIA or NFIA plus SOX9 in hPSCs, we developed a method to efficiently generate astrocytes in 4-7 weeks. The astrocytic identity of the induced cells was verified by their characteristic molecular and functional properties as well as after transplantation. Furthermore, we developed a strategy to generate region-specific astrocyte subtypes by combining differentiation of regional progenitors and transgenic induction of astrocytes. This simple and efficient method offers a new opportunity to study the fundamental biology of human astrocytes and their roles in disease processes.

SUBMITTER: Li X 

PROVIDER: S-EPMC6178885 | biostudies-other | 2018 Oct

REPOSITORIES: biostudies-other

altmetric image

Publications


Differentiation of astrocytes from human pluripotent stem cells (hPSCs) is a tedious and variable process. This hampers the study of hPSC-generated astrocytes in disease processes and drug development. By using CRISPR/Cas9-mediated inducible expression of NFIA or NFIA plus SOX9 in hPSCs, we developed a method to efficiently generate astrocytes in 4-7 weeks. The astrocytic identity of the induced cells was verified by their characteristic molecular and functional properties as well as after trans  ...[more]

Similar Datasets

2018-08-11 | GSE118384 | GEO
| S-EPMC3000364 | biostudies-literature
| PRJNA485416 | ENA
| S-EPMC8586142 | biostudies-literature
| S-EPMC5009184 | biostudies-literature
| S-EPMC4489980 | biostudies-literature
| S-EPMC2799548 | biostudies-literature
| S-EPMC7763297 | biostudies-literature
| S-EPMC4315871 | biostudies-other
| S-EPMC3808201 | biostudies-literature