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Regulation of the G?-cAMP/PKA signaling pathway in cellulose utilization of Chaetomium globosum.

ABSTRACT: BACKGROUND:The canonical heterotrimeric G protein-cAMP/PKA pathway regulates numerous cellular processes in filamentous fungi. Chaetomium globosum, a saprophytic fungus, is known for producing many secondary metabolites, including cytotoxic chaetoglobosin A (ChA), as well as abundant cellulase and xylanase. RESULTS:Here we report on the functional characterization of this signaling pathway in C. globosum. We blocked the pathway by knocking down the putative G?-encoding gene gna1 (in the pG14 mutant). This led to impaired cellulase production and significantly decreased transcription of the major cellulase and xylanase genes. Almost all the glycohydrolase family genes involved in cellulose degradation were downregulated, including the major cellulase genes, cel7a, cel6a, egl1, and egl2. Importantly, the expression of transcription factors was also found to be regulated by gna1, especially Ace1, Clr1/2 and Hap2/3/5 complex. Additionally, carbon metabolic processes including the starch and sucrose metabolism pathway were substantially diminished, as evidenced by RNA-Seq profiling and quantitative reverse transcription (qRT)-PCR. Interestingly, these defects could be restored by simultaneous knockdown of the pkaR gene encoding the regulatory subunit of cAMP-dependent PKA (in the pGP6 mutant) or supplement of the cAMP analog, 8-Br-cAMP. Moreover, the G?-cAMP/PKA pathway regulating cellulase production is modulated by environmental signals including carbon sources and light, in which VelB/VeA/LaeA complex and ENVOY probably work as downstream effectors. CONCLUSION:These results revealed, for the first time, the positive role of the heterotrimeric G?-cAMP/PKA pathway in the regulation of cellulase and xylanase utilization in C. globosum.

SUBMITTER: Hu Y

PROVIDER: S-EPMC6182798 | biostudies-other | 2018 Oct

REPOSITORIES: biostudies-other

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Regulation of the Gα-cAMP/PKA signaling pathway in cellulose utilization of Chaetomium globosum.

Hu Yang Y Liu Yanjie Y Hao Xiaoran X Wang Dan D Akhberdi Oren O Xiang Biyun B Zhu Xudong X

Microbial cell factories 20181011 1

<h4>Background</h4>The canonical heterotrimeric G protein-cAMP/PKA pathway regulates numerous cellular processes in filamentous fungi. Chaetomium globosum, a saprophytic fungus, is known for producing many secondary metabolites, including cytotoxic chaetoglobosin A (ChA), as well as abundant cellulase and xylanase.<h4>Results</h4>Here we report on the functional characterization of this signaling pathway in C. globosum. We blocked the pathway by knocking down the putative Gα-encoding gene gna1 ( ...[more]

PMID: 30309363

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Dataset Information

Regulation of the G?-cAMP/PKA signaling pathway in cellulose utilization of Chaetomium globosum.

Publications

Regulation of the Gα-cAMP/PKA signaling pathway in cellulose utilization of Chaetomium globosum.

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