NDRG2 mRNA levels and miR-28-5p and miR-650 activity in chronic lymphocytic leukemia.

ABSTRACT: BACKGROUND:NDRG2 is identified as a tumor suppressor gene in many tumors, and functions in cell proliferation, differentiation and apoptosis. Recent data indicate that NDRG2 expression is up-regulated by TP53. Moreover, proposed mechanisms of NDRG2 inactivation include epigenetic silencing of the NDRG2 promoter and down-regulation by microRNAs (miRNAs). However, few studies have ever been done on the role of NDRG2 and the NDRG2-regulating miRNAs interference in chronic lymphocytic leukemia (CLL). METHODS:NDRG2 and microRNAs mRNA levels in CLL subjects were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). The dual-luciferase reporter assay was performed to determine NDRG2-related miRNAs. Low expression of mature exogenous miRNAs in CLL cells was established by transient transfection. NDRG2 protein levels in CLL cells were detected by western blot. In addition, flow cytometry was conducted to examine the apoptosis of CLL cells. RESULTS:Lower expression of NDRG2 was found in the B-cells from 102 CLL patients compared the 40 normal subjects (P?

SUBMITTER: Yang YQ 

PROVIDER: S-EPMC6196416 | biostudies-other | 2018 Oct

REPOSITORIES: biostudies-other